The glutathione S-transferase Mu 5 (GSTM5) antibody is a tool used to detect and study the GSTM5 protein, a member of the GST Mu family. GST enzymes play a critical role in cellular detoxification by catalyzing the conjugation of glutathione to electrophilic substrates, aiding in the neutralization of reactive oxygen species, environmental toxins, and carcinogens. The Mu-class GSTs, including GSTM5. are primarily expressed in tissues such as the liver, brain, and testes. GSTM5 is encoded by the *GSTM5* gene located on chromosome 1p13.3 and shares structural homology with other Mu-class isoforms (GSTM1-M4). However, its expression pattern and substrate specificity may differ, suggesting distinct physiological roles. Research on GSTM5 is less extensive compared to other Mu-class GSTs like GSTM1. which is linked to cancer susceptibility and drug metabolism. Antibodies against GSTM5 enable investigations into its tissue distribution, subcellular localization, and interactions in pathways involving oxidative stress, xenobiotic metabolism, and disease mechanisms (e.g., cancer, neurodegenerative disorders). They are vital for techniques like Western blotting, immunohistochemistry, and ELISA. Studying GSTM5 may uncover its potential as a biomarker or therapeutic target, particularly in contexts where GST-mediated detoxification pathways are dysregulated.