HEPH抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10511(HEPH Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

The HEPH antibody is a crucial tool for studying hephaestin (HEPH), a mammalian ferroxidase integral to cellular iron homeostasis. Hephaestin, primarily expressed in intestinal enterocytes and certain brain cells, facilitates iron efflux by oxidizing Fe²⁺ to Fe³⁺, enabling its binding to transferrin for systemic transport. Structurally, HEPH contains multiple copper-binding domains and a transmembrane region, sharing homology with ceruloplasmin, another ferroxidase. Dysregulation of HEPH is linked to iron metabolism disorders, including iron-deficiency anemia and neurodegenerative conditions like Alzheimer’s disease, where iron accumulation exacerbates pathology.

HEPH-specific antibodies are widely used in research to visualize protein localization (e.g., via immunofluorescence or immunohistochemistry) and quantify expression levels (e.g., Western blotting, ELISA). These antibodies, often raised in rabbits or mice, target epitopes within HEPH’s extracellular or cytoplasmic domains. Their applications extend to investigating HEPH’s role in diseases: for instance, intestinal HEPH downregulation impairs dietary iron absorption, while neuronal HEPH dysfunction may contribute to iron-induced oxidative stress. Additionally, HEPH antibodies aid in exploring genetic mutations or regulatory pathways affecting iron export, offering insights into therapeutic strategies for iron-related disorders. Robust validation of these antibodies ensures specificity, minimizing cross-reactivity with homologous proteins like ceruloplasmin.