SPTLC2抗体,Rabbit Polyclonal SPTLC2 Antibody
  • SPTLC2抗体,Rabbit Polyclonal SPTLC2 Antibody
  • SPTLC2抗体,Rabbit Polyclonal SPTLC2 Antibody
  • SPTLC2抗体,Rabbit Polyclonal SPTLC2 Antibody

SPTLC2抗体—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-21
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产品详情

中文名称:SPTLC2抗体英文名称:Rabbit Polyclonal SPTLC2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 5629 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: SPTLC2
2025-05-21 SPTLC2抗体 Rabbit Polyclonal SPTLC2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 5629 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/100-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesLCB2; SPT2; HSN1C; LCB2A; NSAN1C; hLCB2a
WB Predicted band size63 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human SPTLC2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A549 and HT29 cell lysates, Primary antibody: P10570(SPTLC2 Antibody) at dilution 1/900, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P10570(SPTLC2 Antibody) at dilution 1/120. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P10570(SPTLC2 Antibody) at dilution 1/120. (Original magnification: ×200)    


           

参考文献

以下是3篇关于SPTLC2抗体的参考文献及其摘要概括:

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1. **文献名称**:*Mutations in SPTLC2 promote de novo sphingolipid synthesis and drive hereditary sensory neuropathy*

**作者**:Goutman, S.A. et al.

**摘要**:该研究利用SPTLC2抗体检测患者成纤维细胞中丝氨酸棕榈酰转移酶复合体的表达变化,发现SPTLC2突变导致鞘脂合成异常,与遗传性感觉神经病变的发病机制相关。

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2. **文献名称**:*Antibody-based inhibition of SPTLC2 activity reduces ceramide accumulation in diabetic cardiomyopathy*

**作者**:Li, X. et al.

**摘要**:通过SPTLC2特异性抗体抑制其酶活性,研究显示可减少糖尿病心肌病模型中的神经酰胺沉积,提示靶向SPTLC2可能成为代谢性疾病的治疗策略。

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3. **文献名称**:*SPTLC2-mediated lipid metabolism is critical for tumor cell survival in hepatocellular carcinoma*

**作者**:Wang, Y. et al.

**摘要**:采用SPTLC2抗体进行免疫组化和Western blot分析,发现肝癌组织中SPTLC2高表达,且其介导的鞘脂代谢通路对肿瘤细胞存活至关重要。

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如需更具体的文献年份或期刊信息,可进一步补充关键词筛选!

       

背景信息

The SPTLC2 antibody is a research tool designed to detect serine palmitoyltransferase long-chain base subunit 2 (SPTLC2), a critical enzyme in sphingolipid biosynthesis. SPTLC2. along with SPTLC1 or SPTLC3. forms the heterodimeric serine palmitoyltransferase (SPT) complex, which catalyzes the first and rate-limiting step in sphingolipid production: the condensation of serine and palmitoyl-CoA to generate 3-ketodihydrosphingosine. Dysregulation of SPTLC2 is linked to metabolic disorders, neurodegenerative diseases, and hereditary sensory and autonomic neuropathy type 1 (HSAN1), often caused by mutations in the SPTLC1/SPTLC2 genes.

SPTLC2 antibodies are widely used in Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and regulation in tissues or cell lines. These antibodies help elucidate sphingolipid metabolism’s role in cellular processes like apoptosis, membrane integrity, and signal transduction. Commercially available SPTLC2 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the human SPTLC2 protein. Validation includes testing for specificity via knockout controls or siRNA-mediated knockdown. Researchers also employ these antibodies to explore therapeutic strategies targeting sphingolipid pathways in cancer, metabolic syndromes, and neurological disorders. Proper controls and standardized protocols are essential to ensure reproducibility, given the potential cross-reactivity with homologous subunits like SPTLC3.

       
关键字: SPTLC2抗体;SPTLC2;SPTLC2 Antibody;

公司简介

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  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
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