Rat IgG2b-HRP抗体—艾普蒂 新品

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     Lane 1: 100 ng of rat IgG1 Kappa Lane 2: 100 ng of rat IgG2a Kappa Lane 3: 100 ng of rat IgG2b Kappa Lane 4: 100 ng of rat IgG2c Kappa Lane 5: 100 ng of rat IgA Lane 6: 100 ng of rat IgM Lane 7: 100 ng of rat IgG2a Lambda Lane 8: 100 ng of mouse IgG1 Lane 9: 100 ng of mouse IgG2a Lane 10: 100 ng of mouse IgG2b Lane 11: 100 ng of mouse IgG3 Lane 12: 100 ng of mouse IgA Lane 13: 100 ng of mouse IgM Result: P15004 can detect Rat IgG2b by Western blotting.    

  
  

Rat IgG2b-HRP (horseradish peroxidase) antibody is a secondary antibody widely used in immunological assays to detect rat-derived primary antibodies of the IgG2b subclass. Produced in host species like goats or rabbits, it specifically binds to the Fc region of rat IgG2b, enabling targeted detection in techniques such as ELISA, western blotting, and immunohistochemistry. The conjugation of HRP allows colorimetric, chemiluminescent, or fluorescent signal generation upon substrate addition, facilitating visualization of antigen-antibody interactions.

This antibody is valued for its high specificity, minimizing cross-reactivity with other rat IgG subclasses (e.g., IgG1. IgG2a) or immunoglobulins from unrelated species when properly validated. Its applications span biomedical research, including autoimmune studies, infectious disease modeling, and protein expression analysis. Quality control typically includes affinity purification and adsorption steps to reduce non-specific binding. Optimal performance requires titration to balance sensitivity and background noise. As rat IgG2b is less abundant than IgG1 or IgG2a in immune responses, this antibody is particularly useful in models where IgG2b-dominated responses are expected, such as certain vaccine studies or hybridoma-derived monoclonal antibody detection. Proper storage at -20°C with avoidance of freeze-thaw cycles preserves enzymatic activity.