Rat IgG1-HRP抗体—艾普蒂 新品

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     Lane 1: 300 ng of rat IgG1 Lane 2: 300 ng of rat IgG2a Lane 3: 300 ng of rat IgG2b Lane 4: 300 ng of rat IgG2c Lane 5: 300 ng of rat IgA Lane 6: 300 ng of rat IgM Lane 7: 300 ng of mouse IgG2a Lane 8: 300 ng of mouse IgG2b Lane 9: 300 ng of mouse IgA Lane 10: 300 ng of mouse IgGM Lane 11: 300 ng of mouse IgG1 Lane 12: 300 ng of mouse IgG3 Result: P15017 can detect Rat IgG1 by Western blotting.    

  
  

Rat IgG1-HRP antibody is a type of secondary antibody conjugated to horseradish peroxidase (HRP), widely used in immunoassays for detecting rat-derived primary antibodies. Rat IgG1 is a subclass of immunoglobulin G (IgG) commonly produced in rats during immune responses. As a secondary antibody, it specifically binds to the Fc region of rat IgG1 primary antibodies, enabling signal amplification in techniques like Western blotting, ELISA, and immunohistochemistry. The HRP enzyme catalyzes colorimetric, chemiluminescent, or fluorescent reactions when exposed to substrates, allowing visualization of target proteins or antigens.

These antibodies are typically raised in hosts like goats or rabbits immunized with rat IgG1. followed by purification and HRP conjugation. Their specificity is validated to minimize cross-reactivity with immunoglobulins from other species or IgG subclasses. Rat IgG1-HRP antibodies are essential in dual-labeling experiments or when working with rat monoclonal primary antibodies, particularly in immunology and cancer research. Quality control ensures batch-to-batch consistency, high sensitivity, and low background noise. Proper storage at -20°C and avoidance of repeated freeze-thaw cycles are critical for maintaining activity. Their versatility and reliability make them indispensable tools in biomedical research and diagnostic applications.