The GCG gene encodes proglucagon, a precursor protein that is post-translationally processed into several bioactive peptides, including glucagon-like peptide-2 (GLP-2). GLP-2 is a 33-amino-acid hormone primarily secreted by intestinal enteroendocrine L-cells in response to nutrient intake. It plays a critical role in regulating intestinal homeostasis by promoting mucosal growth, enhancing nutrient absorption, and reducing epithelial permeability. GLP-2 also exhibits anti-inflammatory properties and supports gut barrier function. Antibodies targeting GLP-2 (GLP-2 antibodies) are essential tools for studying its physiological functions, distribution, and receptor interactions. They are widely used in immunoassays (e.g., ELISA, immunohistochemistry) to quantify GLP-2 levels in research on metabolic disorders, gastrointestinal diseases (e.g., short bowel syndrome, inflammatory bowel disease), and obesity. Additionally, GLP-2 antibodies have therapeutic potential, as neutralizing antibodies may help investigate pathologies linked to GLP-2 dysregulation, while agonist analogs (e.g., teduglutide) are already clinically used to treat intestinal insufficiency. Research continues to explore GLP-2's role in gut-brain signaling and its broader metabolic impacts, underscoring the importance of specific antibodies in both mechanistic studies and diagnostic applications.