LAMTOR1 (Late Endosomal/Lysosomal Adaptor, MAPK and mTOR Activator 1), also known as p18 or Ragulator1. is a critical component of the Ragulator complex that anchors the mTORC1 signaling pathway to lysosomal membranes. This protein plays a pivotal role in nutrient sensing, cellular metabolism, and autophagy by regulating mTORC1 activation in response to amino acid availability. Structurally, LAMTOR1 contains a lipid-binding domain that facilitates its association with lysosomal membranes and serves as a scaffold for assembling other Ragulator subunits (LAMTOR2-5).
Antibodies targeting LAMTOR1 are essential tools for studying its function and interactions in mTOR signaling. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, subcellular localization, and complex formation. High-quality LAMTOR1 antibodies exhibit specificity for distinct epitopes, often validated through knockout or knockdown controls, ensuring reliability in experimental models.
Research applications span cancer biology, neurodegenerative diseases, and metabolic disorders, as dysregulation of LAMTOR1 is linked to aberrant mTOR signaling in these conditions. Additionally, these antibodies aid in exploring therapeutic targets by elucidating how LAMTOR1-mediated lysosomal positioning and nutrient sensing influence cell growth and survival. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) are selected based on experimental requirements, balancing sensitivity and specificity. Proper validation under specific experimental conditions remains crucial for reproducible results.