The FOXQ1 antibody is a crucial tool for studying the FOXQ1 protein, a member of the Forkhead box (FOX) transcription factor family. FOXQ1. encoded by the *FOXQ1* gene, regulates gene expression by binding to DNA via its conserved forkhead domain. It plays pivotal roles in embryonic development, cell differentiation, and tissue homeostasis. Recent research highlights its involvement in cancer progression, particularly in epithelial-mesenchymal transition (EMT), metastasis, and chemoresistance. Overexpression of FOXQ1 is linked to aggressive phenotypes in colorectal, breast, and gastric cancers, making it a potential prognostic biomarker and therapeutic target.
FOXQ1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect FOXQ1 expression and localization in tissues or cell lines. These antibodies aid in elucidating FOXQ1's molecular mechanisms, such as its interaction with Wnt/β-catenin and TGF-β signaling pathways. Commercial FOXQ1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal regions). Validation includes knockout cell controls and recombinant protein assays to ensure specificity.
Research utilizing FOXQ1 antibodies continues to expand, offering insights into its dual roles as an oncogene or tumor suppressor, depending on context. This underscores the antibody's importance in both basic research and clinical applications.