The GTF3C3 antibody is a tool used to detect and study the General Transcription Factor III C Subunit 3 (GTF3C3), a protein component of the RNA polymerase III (Pol III) transcription factor complex. GTF3C3. along with other GTF3C subunits (GTF3C1-6), plays a critical role in the assembly and recruitment of the Pol III machinery to target genes, including those encoding small non-coding RNAs like tRNAs, 5S rRNA, and other essential small RNAs. This factor is vital for transcription initiation, as it binds to promoters and facilitates the proper positioning of Pol III for RNA synthesis.
Antibodies targeting GTF3C3 are primarily utilized in research to investigate its expression, localization, and interaction partners in cellular contexts. They are commonly applied in techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Studies involving GTF3C3 antibodies have contributed to understanding dysregulation in Pol III-dependent transcription, which is implicated in diseases like cancer, viral infections, and neurodevelopmental disorders. For example, altered Pol III activity has been observed in tumorigenesis, where uncontrolled cell proliferation demands elevated RNA synthesis.
Commercial GTF3C3 antibodies are typically validated for specificity in model systems (e.g., human, mouse), though cross-reactivity may vary. Researchers rely on these antibodies to explore transcriptional mechanisms, gene regulation, and potential therapeutic targets linked to Pol III dysfunction.