The RNF133 antibody is a research tool designed to detect and study the RNF133 protein, a member of the ring finger (RNF) family of E3 ubiquitin ligases. RNF133 is a transmembrane protein localized primarily to the endoplasmic reticulum (ER), where it is implicated in ER-associated degradation (ERAD) and ubiquitin-proteasome pathways. It plays a role in protein quality control by tagging misfolded proteins for proteasomal degradation. Research suggests RNF133 may regulate cellular processes such as apoptosis, receptor trafficking, and immune responses. Dysregulation of RNF133 has been linked to cancers, including colorectal and gastric cancers, where its expression may influence tumor progression or suppression. The antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate RNF133’s expression patterns, interactions, and functional mechanisms. Due to its specificity for RNF133. the antibody aids in distinguishing its activity from other RNF family members, which share structural similarities but have distinct biological roles. Current studies focus on clarifying its dual roles in oncogenesis and its potential as a therapeutic target. Validation of RNF133 antibodies typically includes knockout cell controls to confirm specificity, ensuring reliability in experimental models.