α-Tubulin抗体;α-Tubulin Antibody—艾普蒂

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     Lane 1: 15 µg of A-431 lysate;  Lane 2: 15 µg of U-251MG lysate;  Lane 3: 15 µg of HeLa lysate;Lane 4: 15 µg of Hep G2 lysate;  Result: P15819 can detect α-Tubulin by Western blotting.    

  
  

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     IHC-P analysis of testis tissue by TUBA4A antibody (P15819) IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue;Result: Cells in seminiferous ducts and Leydig cells are positively stained at cytoplasm and membrane.    

  
  

α-Tubulin is a core component of microtubules, dynamic cytoskeletal polymers essential for eukaryotic cell structure, division, motility, and intracellular transport. It belongs to the tubulin superfamily and forms heterodimers with β-tubulin, which assemble into protofilaments that constitute microtubules. α-Tubulin plays critical roles in mitosis, organelle positioning, and cell shape maintenance. Its expression is generally stable across cell types, making it a widely used loading control in Western blotting and other protein analysis techniques to normalize sample quantities.

Antibodies targeting α-tubulin are indispensable tools in cell biology research. They enable visualization of microtubule networks via immunofluorescence, aiding studies on cell cycle dynamics, cytoskeletal organization, and drug effects (e.g., microtubule-targeting chemotherapeutics). Commonly used clones like DM1A recognize conserved epitopes, ensuring cross-reactivity in human, mouse, rat, and other model organisms. These antibodies also detect post-translational modifications (e.g., acetylation, detyrosination) linked to microtubule stability and function.

In disease research, α-tubulin antibodies help investigate pathologies involving cytoskeletal defects, such as neurodegenerative disorders and cancer. Their reliability as housekeeping markers ensures accurate data interpretation in protein expression studies, underscoring their versatility in both basic and applied biomedical research.