Phospho-AMPKalpha(Thr172)抗体;Phospho-AMPKalpha(Thr172) Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Phospho-AMPK alpha (T172) expression in Mouse heart lysate.    

  
  

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     Salidroside attenuates hypoxia-induced pulmonary arterial smooth muscle cell proliferation and apoptosis resistance by upregulating autophagy through the AMPK-mTOR-ULK1 pathway. -BMC Pulmonary Medicine    

  
  

The Phospho-AMPKα (Thr172) antibody is a critical tool for studying the activation status of AMP-activated protein kinase (AMPK), a central regulator of cellular energy homeostasis. AMPK, a heterotrimeric enzyme composed of α, β, and γ subunits, is activated under low-energy conditions (e.g., ATP depletion) via phosphorylation at Thr172 within the α subunit’s catalytic domain. This phosphorylation, mediated by upstream kinases like LKB1 or CaMKKβ, is essential for AMPK’s ability to restore energy balance by inhibiting anabolic pathways (e.g., lipid/cholesterol synthesis) and promoting catabolic processes (e.g., glycolysis, fatty acid oxidation).

The Phospho-AMPKα (Thr172) antibody specifically detects AMPKα phosphorylated at Thr172. serving as a marker for AMPK activation. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate AMPK signaling in metabolic disorders (e.g., diabetes, obesity), cancer, aging, and exercise physiology. Researchers employ this antibody to assess AMPK activity in response to stimuli such as nutrient deprivation, pharmacological agents (e.g., metformin, AICAR), or genetic manipulations.

Validation often includes testing in AMPK-deficient models or after treatment with AMPK activators/inhibitors to confirm specificity. Cross-reactivity with related kinases (e.g., phosphorylated Akt) should be ruled out. Its applications extend to preclinical studies evaluating therapeutic strategies targeting AMPK pathways. This antibody has become indispensable for elucidating AMPK’s role in health and disease, bridging molecular mechanisms to physiological outcomes.