Argonaute2抗体,Rabbit Monoclonal Argonaute2 Antibody
  • Argonaute2抗体,Rabbit Monoclonal Argonaute2 Antibody
  • Argonaute2抗体,Rabbit Monoclonal Argonaute2 Antibody
  • Argonaute2抗体,Rabbit Monoclonal Argonaute2 Antibody

Argonaute2抗体;Argonaute2 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-29
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产品详情

中文名称:Argonaute2抗体英文名称:Rabbit Monoclonal Argonaute2 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8028 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: Argonaute2
2025-05-29 Argonaute2抗体 Rabbit Monoclonal Argonaute2 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8028 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20-1/50 Human,Mouse,Rat
IHCIHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM1/20-1/100 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesAgo 2; Argonaute 2; dAgo2; eIF2C 2; hAgo2; PPD; ;Argonaute 2
WB Predicted band size97 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Argonaute 2
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    


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       Western blot analysis of Argonaute 2 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.    


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       Immunofluorescent analysis using the Antibody at 1:50 dilution.    


           

参考文献

以下是关于Argonaute2抗体的3-4篇参考文献的简要列举:

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1. **文献名称**:*Argonaute2 Is the Catalytic Engine of Mammalian RNAi*

**作者**:Meister, G., et al.

**摘要**:该研究通过免疫沉淀和功能实验,证实Argonaute2(Ago2)是哺乳动物RNA干扰(RNAi)途径的核心催化组分,其抗体被用于鉴定Ago2在RNA诱导沉默复合体(RISC)中的特异性作用。

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2. **文献名称**:*Structural Basis for Double-Stranded RNA Processing by Dicer*

**作者**:Liu, J., et al.

**摘要**:通过结构生物学手段解析Ago2与其他RNAi相关蛋白的相互作用,研究中利用Ago2抗体进行免疫共沉淀实验,揭示了其在siRNA引导的基因沉默中的关键结构特征。

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3. **文献名称**:*A Human, ATP-Independent, RISC Assembly Machine Fueled by pre-miRNA*

**作者**:Ender, C., et al.

**摘要**:该文献探讨了Ago2在microRNA(miRNA)介导的基因沉默中的功能,通过特异性抗体验证了Ago2在RISC复合体中的核心地位及其对miRNA稳定性的影响。

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4. **文献名称**:*Specificity and Cross-Reactivity of Argonaute Antibodies in Immunoprecipitation*

**作者**:Schürmann, N., et al.

**摘要**:系统评估了多种商业Ago2抗体的特异性及交叉反应性,强调了抗体选择对免疫沉淀(IP)和Western blot实验结果可靠性的重要性。

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以上文献覆盖了Ago2的功能机制、结构研究、抗体应用验证等方向,可作为相关实验设计的参考。

       

背景信息

Argonaute2 (AGO2) is a critical protein component of the RNA-induced silencing complex (RISC), a central machinery in post-transcriptional gene regulation. It plays a pivotal role in small RNA-guided gene silencing pathways, including microRNA (miRNA)-mediated mRNA degradation/translation repression and small interfering RNA (siRNA)-driven RNA interference (RNAi). AGO2 binds to small RNA molecules (miRNAs or siRNAs) and uses them as guides to target complementary mRNA sequences, leading to gene silencing through mRNA cleavage or recruitment of additional silencing factors.

AGO2 antibodies are essential tools for studying its expression, localization, and function in diverse biological contexts. These antibodies enable researchers to detect AGO2 protein levels via Western blotting, immunoprecipitation (IP), or immunofluorescence (IF), and to investigate its interactions with other RISC components or target mRNAs. Specific AGO2 antibodies can distinguish between its active (e.g., phosphorylated) and inactive states, providing insights into regulatory mechanisms. They are widely used in cancer research, neurobiology, and developmental studies due to AGO2's involvement in cell proliferation, differentiation, and disease pathways like tumorigenesis or neurodegeneration.

Commercial AGO2 antibodies are typically raised against epitopes in its N-terminal or PIWI domains, with validation in knockout controls to ensure specificity. Cross-reactivity varies; many detect human, mouse, and rat homologs. Proper validation is critical, as off-target binding to other Argonaute family members (AGO1. AGO3. AGO4) may occur. These reagents continue to advance our understanding of RNA-based gene regulation and therapeutic applications of RNAi.

       
关键字: Argonaute2抗体;Argonaute2;Argonaute2 Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
普通会员
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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