The Flap Endonuclease 1 (FEN1) antibody is a crucial tool for studying the role of FEN1. a highly conserved structure-specific nuclease essential in DNA replication and repair. FEN1 plays a pivotal role in processing Okazaki fragments during lagging-strand DNA synthesis by cleaving 5' overhanging flaps, ensuring genomic stability. It also participates in long-patch base excision repair (BER) and stalled replication fork rescue. Dysregulation of FEN1 is linked to cancer, neurodegenerative disorders, and aging, making it a key focus in disease research.
FEN1 antibodies are widely used to detect FEN1 protein expression, localization, and interactions in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). These antibodies are typically raised in hosts such as rabbits or mice, targeting specific epitopes (e.g., human FEN1 amino acid residues 50-150). Validated applications and species reactivity (human, mouse, rat) are critical considerations when selecting FEN1 antibodies.
High-quality FEN1 antibodies exhibit specificity confirmed by knockout (KO) validation or siRNA knockdown, ensuring minimal cross-reactivity. They enable researchers to explore FEN1's biological functions, its overexpression in certain cancers (e.g., breast, lung), and its potential as a therapeutic target. Commercial availability from major suppliers (e.g., Abcam, Cell Signaling Technology) ensures accessibility for diverse experimental needs. Proper controls, like isotype-matched IgG and FEN1-deficient cell lysates, are essential for accurate data interpretation.