**Background of PCBP1 Antibody**
PCBP1 (Poly(rC)-Binding Protein 1), also known as hnRNP E1 or αCP1. is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It is a multifunctional RNA-binding protein involved in post-transcriptional gene regulation, including mRNA stability, translation, and splicing. PCBP1 binds to poly(rC)-rich sequences in target RNAs through its KH (K-homology) domains, facilitating interactions with transcripts critical for cellular processes like iron homeostasis, stress responses, and viral replication.
PCBP1 antibodies are essential tools for studying the protein's expression, localization, and functional roles. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate PCBP1's involvement in diseases like cancer, neurodegenerative disorders, and iron metabolism dysregulation. For instance, PCBP1 dysregulation has been linked to tumor progression due to its role in modulating oncogenic or tumor-suppressive mRNAs. Additionally, PCBP1 participates in cellular iron management by stabilizing ferritin mRNA and regulating iron-responsive element (IRE)-binding activity.
These antibodies are also valuable in exploring PCBP1's interaction with viral RNAs or its role in stress granule formation during cellular stress. Validated for specificity and sensitivity, PCBP1 antibodies enable researchers to dissect molecular mechanisms underlying gene expression control and disease pathogenesis.