**Background of Phospho-NAK/TBK1(S172) Antibody**
The Phospho-NAK/TBK1 (Ser172) antibody is a specialized tool used to detect the activated form of TANK-binding kinase 1 (TBK1), also known as NAK (NF-κB activating kinase). TBK1 is a serine/threonine kinase belonging to the IκB kinase (IKK) family, playing a critical role in innate immune responses, particularly in signaling pathways triggered by pathogen-associated molecular patterns (PAMPs) or damage-associated molecular patterns (DAMPs).
Activation of TBK1 occurs via phosphorylation at Ser172. a key regulatory site within its activation loop. This phosphorylation event is essential for TBK1 dimerization, autophosphorylation, and subsequent activation of downstream effectors, such as interferon regulatory factors (IRF3/IRF7), which drive type I interferon (IFN-α/β) production. The Phospho-TBK1(S172) antibody specifically recognizes this phosphorylated form, enabling researchers to study TBK1 activation dynamics in immune signaling pathways like the STING, RIG-I, and TLR pathways.
This antibody is widely used in immunoblotting (Western blot), immunofluorescence, and immunoprecipitation to investigate TBK1’s role in viral infections, autoimmune disorders, and cancer. Dysregulation of TBK1 activity has been linked to diseases such as lupus, rheumatoid arthritis, and certain malignancies, making this antibody a valuable tool for both basic research and therapeutic development. Its specificity ensures accurate detection of TBK1 activation status, aiding in the elucidation of molecular mechanisms underlying immune regulation and disease pathogenesis.