Acetyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
  • Acetyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
  • Acetyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
  • Acetyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody

Acetyl-Histone H3 (Lys27)抗体;Acetyl-Histone H3 (Lys27) Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
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产品详情

中文名称:Acetyl-Histone H3 (Lys27)抗体英文名称:Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8896 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: Acetyl-Histone H3 (Lys27)
2025-06-03 Acetyl-Histone H3 (Lys27)抗体 Rabbit Monoclonal Acetyl-HistoneH3(Lys27) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8896 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesH3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
Entrez GeneID8350
WB Predicted band sizeCalculated MW: 15 kDa; Observed MW: 15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic acetyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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       Western blot analysis of Histone H3 (acetylLys27) in Hela, A549, HL-60 lysates using Acetyl-Histone H3 (Lys27) antibody.    


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       Immunocytochemistry analysis of Histone H3 (acetylLys27) (green) in hela using Histone H3 (acetylLys27) antibody,and DAPI(blue)    


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       Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Histone H3 (acetylLys27) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    


           

参考文献

以下是关于Acetyl-Histone H3 (Lys27)抗体的3篇参考文献,按文献名称、作者和摘要内容分类整理:

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1. **文献名称**: "Histone H3K27ac separates active from poised enhancers and predicts developmental state"

**作者**: Creyghton, M.P. 等

**摘要**: 该研究利用H3K27ac抗体通过ChIP-seq技术,区分了活性增强子与静息态增强子,发现H3K27ac标记与细胞发育阶段密切相关,为表观遗传调控提供了关键分子标志。

2. **文献名称**: "Global mapping of H3K27ac in cancer reveals oncogenic reprogramming of enhancer landscapes"

**作者**: Zhang, X. 等

**摘要**: 研究通过H3K27ac抗体分析多种癌症模型,揭示了肿瘤细胞中增强子区域乙酰化异常,表明H3K27ac在癌基因激活和肿瘤发生中起重要作用。

3. **文献名称**: "Dynamic acetylation of histone H3K27 marks developmental transitions in mammalian chromatin"

**作者**: Tie, F. 等

**摘要**: 该文献使用H3K27ac抗体探究胚胎干细胞分化过程中组蛋白修饰的动态变化,发现其与关键发育基因的时序性激活直接相关,为细胞命运决定提供机制解释。

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这些研究均通过H3K27ac抗体揭示该修饰在基因调控、疾病机制及发育生物学中的功能,展示了该抗体在染色质分析中的广泛应用。

       

背景信息

The Acetyl-Histone H3 (Lys27) antibody is a key tool for studying epigenetic regulation, specifically targeting the acetylation of lysine 27 on histone H3 (H3K27ac). Histone acetylation is a post-translational modification critical for chromatin structure and gene expression regulation. By neutralizing the positive charge of lysine residues, acetylation reduces histone-DNA interactions, promoting an open chromatin state that facilitates transcriptional activation. H3K27ac is enriched at active enhancers and promoters, serving as a hallmark of regulatory elements involved in cell-specific gene programs. Unlike H3K27 methylation (e.g., H3K27me3. associated with Polycomb-mediated gene silencing), H3K27ac is linked to transcriptional activation and is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs).

This antibody is widely used in chromatin immunoprecipitation (ChIP) assays to map genomic regions associated with active transcription, as well as in techniques like Western blotting and immunofluorescence to assess acetylation levels in various biological contexts. Dysregulation of H3K27ac has been implicated in diseases such as cancer, where aberrant enhancer activity drives oncogene expression, and in neurological disorders. Researchers rely on validated Acetyl-Histone H3 (Lys27) antibodies to explore epigenetic mechanisms, evaluate drug effects targeting acetylation pathways, and identify biomarkers for disease diagnosis or therapy. Its specificity and reliability make it essential for studies linking chromatin dynamics to cellular function and disease.

       
关键字: Acetyl-Histone H3 (L;Acetyl-Histone H3 (L;Acetyl-Histone H3 (L;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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