TriMethyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal TriMethyl-HistoneH3(Lys27) Antibody
  • TriMethyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal TriMethyl-HistoneH3(Lys27) Antibody
  • TriMethyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal TriMethyl-HistoneH3(Lys27) Antibody
  • TriMethyl-Histone H3 (Lys27)抗体,Rabbit Monoclonal TriMethyl-HistoneH3(Lys27) Antibody

TriMethyl-Histone H3 (Lys27)抗体;TriMethyl-Histone H3 (Lys27) Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
QQ交谈 微信洽谈

产品详情

中文名称:TriMethyl-Histone H3 (Lys27)抗体英文名称:Rabbit Monoclonal TriMethyl-HistoneH3(Lys27) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8913 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: TriMethyl-Histone H3 (Lys27)
2025-06-03 TriMethyl-Histone H3 (Lys27)抗体 Rabbit Monoclonal TriMethyl-HistoneH3(Lys27) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8913 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesH3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
Entrez GeneID8350
WB Predicted band sizeCalculated MW: 15 kDa; Observed MW: 15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Rat
ImmunogenA synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

+ +

  •  

     

       Western blot analysis of Histone H3 (tri methylLys27) in C6, Jurkat lysates using TriMethyl-Histone H3 (Lys27) antibody.    


  •  

     

       Immunocytochemistry analysis of TriMethyl-Histone H3 (Lys27) (green) in Hela using TriMethyl-Histone H3 (Lys27) antibody,and DAPI(blue)    


  •  

     

       Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TriMethyl-Histone H3 (Lys27) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    


           

参考文献

以下是关于TriMethyl-Histone H3 (Lys27)抗体的3篇参考文献及其简要摘要:

1. **文献名称**:*Role of histone H3 lysine 27 methylation in Polycomb-group silencing*

**作者**:Cao, R. et al.

**摘要**:该研究揭示了H3K27的三甲基化(H3K27me3)是Polycomb抑制复合体(PRC2)介导基因沉默的关键表观遗传标记,并证实了特异性抗体在染色质免疫沉淀(ChIP)中的应用,为研究发育和癌症中的基因调控提供了工具。

2. **文献名称**:*Polycomb complexes repress developmental regulators in murine embryonic stem cells*

**作者**:Boyer, L.A. et al.

**摘要**:文章通过H3K27me3抗体的ChIP-seq分析,证明Polycomb复合体在胚胎干细胞中通过H3K27三甲基化抑制发育相关基因,维持干细胞多能性,为表观遗传调控机制提供了实验依据。

3. **文献名称**:*SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex*

**作者**:Pasini, D. et al.

**摘要**:研究利用H3K27me3抗体验证了SUZ12在PRC2复合体中的必要性,表明其缺失导致H3K27三甲基化水平下降及靶基因异常激活,强调了该抗体在解析PRC2功能中的关键作用。

如需获取全文,建议通过PubMed或Sci-Hub等平台检索文献标题及作者。

       

背景信息

TriMethyl-Histone H3 (Lys27) [H3K27me3] antibodies are essential tools for studying post-translational modifications associated with epigenetic regulation. Histone H3 lysine 27 trimethylation is a conserved marker of transcriptionally repressive chromatin, primarily catalyzed by Polycomb repressive complex 2 (PRC2), with EZH2 or EZH1 as the catalytic subunit. This modification plays a critical role in gene silencing, X-chromosome inactivation, and maintenance of stem cell pluripotency. Aberrant H3K27me3 levels are linked to developmental disorders and cancers, such as gliomas, where H3K27M mutations drive oncogenesis, and certain solid tumors with PRC2 dysregulation.

H3K27me3-specific antibodies are widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to map repressive domains, assess PRC2 activity, or investigate crosstalk with activating marks like H3K4me3. They are critical for studying cellular differentiation, tumor progression, and epigenetic therapies targeting PRC2 (e.g., EZH2 inhibitors). Validation in knockout models or enzymatic demethylation controls is recommended due to potential cross-reactivity with other methylated residues. These antibodies also aid in diagnosing H3K27-altered cancers, highlighting their dual research and clinical utility.

       
关键字: TriMethyl-Histone H3;TriMethyl-Histone H3;TriMethyl-Histone H3;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

TriMethyl-Histone H3 (Lys27)抗体;TriMethyl-Histone H3 (Lys27) Antibody—艾普蒂相关厂家报价

产品名称 价格   公司名称 报价日期
¥1600.00
VIP10年
北京索莱宝科技有限公司
2026-01-30
¥4770
武汉艾美捷科技有限公司
2026-01-29
询价
碧云天生物技术有限公司
2026-01-29
内容声明:
以上所展示的信息由商家自行提供,内容的真实性、准确性和合法性由发布商家负责。 商家发布价格指该商品的参考价格,并非原价,该价格可能随着市场变化,或是由于您购买数量不同或所选规格不同而发生变化。最终成交价格,请咨询商家,以实际成交价格为准。请意识到互联网交易中的风险是客观存在的
主页 | 企业会员服务 | 广告业务 | 联系我们 | 旧版入口 | 中文MSDS | CAS Index | 常用化学品CAS列表 | 化工产品目录 | 新产品列表 |投诉中心
Copyright © 2008 ChemicalBook 京ICP备07040585号  京公海网安备110108000080号  All rights reserved.