Acetyl-p53 (Lys370)抗体;Acetyl-p53 (Lys370) Antibody—艾普蒂

+ +

•  

   

     Western blot analysis of p53 (acetyl K370) in HeLa lysates treated with Trichostatin A using Acetyl-p53 (Lys370) antibody.    

  
  

The Acetyl-p53 (Lys370) antibody is a specialized tool used to study post-translational modifications of the tumor suppressor protein p53. a critical regulator of cell cycle arrest, DNA repair, and apoptosis. p53 becomes activated in response to cellular stress, such as DNA damage, and its activity is tightly controlled by modifications including phosphorylation, ubiquitination, and acetylation. Acetylation of p53 at lysine 370 (K370) is a key regulatory event mediated by histone acetyltransferases (HATs) like p300/CBP, which enhance p53's transcriptional activity by promoting chromatin relaxation and co-activator recruitment. This modification stabilizes p53 by reducing its interaction with the negative regulator MDM2 and enhances its ability to bind target gene promoters.

The Acetyl-p53 (K370) antibody specifically detects p53 acetylated at this residue, enabling researchers to investigate the activation status and functional regulation of p53 in various contexts, including cancer, where p53 mutations or dysregulated acetylation are common. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study DNA damage responses, epigenetic regulation, and therapeutic interventions targeting p53 pathways. By focusing on K370 acetylation, this antibody provides insights into how post-translational modifications fine-tune p53's tumor-suppressive functions, offering a valuable tool for understanding cancer biology and developing precision therapies.