The MonoMethyl-Histone H2B (Arg79) antibody is a specialized tool used to detect monomethylation at arginine 79 (Arg79) of histone H2B, a post-translational modification (PTM) critical for epigenetic regulation. Histones, including H2B, are core components of nucleosomes, which organize DNA into chromatin. PTMs like methylation dynamically influence chromatin structure and gene expression by modulating interactions between histones, DNA, and regulatory proteins.
Arg79 methylation in H2B is less characterized compared to other histone modifications but is implicated in transcriptional regulation, DNA repair, and chromatin remodeling. Methylation at this site may interact with reader proteins to recruit chromatin-modifying complexes or influence nucleosome stability. Studies suggest crosstalk between H2B Arg79 methylation and other histone marks, such as H3K4me3 or H4K20me, highlighting its role in maintaining epigenetic balance.
This antibody is essential for techniques like Western blotting, ChIP-seq, or immunofluorescence to map methylation patterns, study its biological roles, or explore dysregulation in diseases like cancer or developmental disorders. Specificity validation (e.g., using methylated peptides or knockout models) ensures accurate detection. Research using this antibody contributes to understanding how H2B Arg79 monomethylation fine-tunes chromatin dynamics and cellular processes, bridging gaps in epigenetic mechanisms.