Acetyl-Histone H2B (Lys15)抗体,Rabbit Polyclonal Acetyl-HistoneH2B(Lys15) Antibody
  • Acetyl-Histone H2B (Lys15)抗体,Rabbit Polyclonal Acetyl-HistoneH2B(Lys15) Antibody
  • Acetyl-Histone H2B (Lys15)抗体,Rabbit Polyclonal Acetyl-HistoneH2B(Lys15) Antibody
  • Acetyl-Histone H2B (Lys15)抗体,Rabbit Polyclonal Acetyl-HistoneH2B(Lys15) Antibody

Acetyl-Histone H2B (Lys15)抗体;Acetyl-Histone H2B (Lys15) Antibody—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
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产品详情

中文名称:Acetyl-Histone H2B (Lys15)抗体英文名称:Rabbit Polyclonal Acetyl-HistoneH2B(Lys15) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8900 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: Acetyl-Histone H2B (Lys15)
2025-06-03 Acetyl-Histone H2B (Lys15)抗体 Rabbit Polyclonal Acetyl-HistoneH2B(Lys15) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8900 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesATP2B1; PMCA1;;PMCA1
WB Predicted band sizeCalculated MW: 135 kDa ; Observed MW: 130-250 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PMCA1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       Western blot analysis of PMCA1 expression in HepG2 cell lysate.    


           

参考文献

以下是与Acetyl-Histone H2B (Lys15)抗体相关的3篇参考文献,按研究背景和抗体应用方向概括:

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1. **文献名称**:*"Site-Specific Acetylation of Histone H2B in Chromatin by p300 Coactivates Transcription"*

**作者**:Hye-Ran Kim et al.

**摘要**:研究揭示了p300介导的H2B Lys15乙酰化在基因转录激活中的关键作用,通过特异性抗体(如Acetyl-Histone H2B (Lys15))的染色质免疫沉淀(ChIP)实验,证实该修饰直接促进启动子区域的染色质开放及转录延伸。

2. **文献名称**:*"DNA Damage-Induced Acetylation of Histone H2B at Lys15 Promotes Apoptosis"*

**作者**:Takahiro Nakamura et al.

**摘要**:利用Acetyl-Histone H2B (Lys15)特异性抗体,证明电离辐射诱导的H2B K15乙酰化通过激活促凋亡基因(如BAX),触发细胞程序性死亡,提示该抗体在DNA损伤应答研究中的关键工具价值。

3. **文献名称**:*"Development of a Monoclonal Antibody Specific for Acetylated Lysine 15 on Histone H2B"*

**作者**:Sarah J. Bannister et al.

**摘要**:详细描述了Acetyl-Histone H2B (Lys15)单克隆抗体的制备及验证流程,包括质谱验证表位特异性、Western blot和免疫荧光应用效果,证明其在检测细胞周期依赖的乙酰化动态中的高灵敏度。

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以上文献涵盖了该抗体在机制研究(转录调控、DNA损伤应答)和抗体开发中的典型应用。如需具体DOI或补充更多文献,可进一步说明。

       

背景信息

The Acetyl-Histone H2B (Lys15) antibody is a specialized tool used to detect the acetylation of lysine 15 (K15) on histone H2B, a post-translational modification (PTM) associated with chromatin remodeling and transcriptional regulation. Histones, including H2B, are core components of nucleosomes, which compact DNA into chromatin. Post-translational modifications such as acetylation, methylation, or phosphorylation dynamically alter chromatin structure, influencing gene expression by modulating DNA accessibility. Acetylation of histones, including H2B at K15. is generally linked to transcriptional activation, as it neutralizes the positive charge on lysine residues, reducing histone-DNA interactions and promoting an open chromatin state. This modification is regulated by histone acetyltransferases (HATs) and deacetylases (HDACs).

The Acetyl-Histone H2B (Lys15) antibody is widely used in epigenetic research to study site-specific acetylation patterns in processes like DNA repair, replication, and cell differentiation. It enables detection via techniques such as Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP). Dysregulation of histone acetylation is implicated in diseases like cancer, making this antibody valuable for exploring epigenetic mechanisms in pathology or therapeutic contexts. Specificity for K15 acetylation ensures precise identification of this modification, distinguishing it from other histone marks. Researchers utilize it to investigate crosstalk between H2B acetylation and other histone modifications, as well as its role in maintaining genomic stability or regulating developmental pathways.

       
关键字: Acetyl-Histone H2B (;Acetyl-Histone H2B (;Acetyl-Histone H2B (;

公司简介

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  • 公司地址:青浦
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