The Phospho-PTP1B (Ser378) antibody is designed to detect protein tyrosine phosphatase 1B (PTP1B) when phosphorylated at serine residue 378. a post-translational modification critical for regulating its enzymatic activity. PTP1B, encoded by the *PTPN1* gene, is a key regulator of metabolic signaling pathways, including insulin and leptin receptor signaling. It dephosphorylates receptor tyrosine kinases (e.g., insulin receptor, EGFR) and downstream effectors, thereby attenuating signaling cascades. Phosphorylation at Ser378. mediated by kinases such as AKT or ERK, induces a conformational change that inhibits PTP1B activity, enhancing downstream signaling. This modification is implicated in metabolic disorders (e.g., diabetes, obesity) and cancer progression.
The Phospho-PTP1B (Ser378) antibody enables researchers to study the activation status of PTP1B in cellular contexts, particularly under conditions of insulin resistance, oxidative stress, or oncogenic signaling. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess phosphorylation-dependent PTP1B regulation in tissues or cultured cells. Validated for specificity, this antibody helps elucidate mechanisms linking PTP1B modulation to disease pathways, offering insights for therapeutic targeting. Its application spans diabetes research, cancer biology, and studies on endoplasmic reticulum stress, where PTP1B’s role in protein folding and degradation is increasingly recognized.