Phospho-PTS (Ser19)抗体;Phospho-PTS (Ser19) Antibody—艾普蒂

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     Western blot analysis of PTS/PTPS (Phospho-Ser19) in A549, HL-60 lysates using Phospho-PTS (Ser19) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-PTS/PTPS (Ser19) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The Phospho-PTS (Ser19) antibody is a specific tool used to detect phosphorylation of the PTS (phosphotyrosine substrate) protein at serine residue 19. PTS, also known as STYX (serine/threonine/tyrosine-interacting protein), is a pseudophosphatase involved in regulating cellular signaling pathways, including MAPK (mitogen-activated protein kinase) cascades. Unlike active phosphatases, PTS lacks catalytic activity due to critical amino acid substitutions, but it binds phosphorylated targets, modulating their activity or localization. Phosphorylation at Ser19 is thought to influence PTS interactions with signaling components, impacting processes like cell cycle regulation, stress responses, and apoptosis.

This antibody enables researchers to study the activation status and functional role of PTS in various contexts, such as cancer, neuronal development, or metabolic disorders. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess phosphorylation-dependent protein interactions or pathway activation. Validation often includes testing in knockout cell lines or phosphatase-treated samples to confirm specificity.

Understanding PTS phosphorylation dynamics provides insights into how pseudophosphatases fine-tune signaling networks without enzymatic activity. The Phospho-PTS (Ser19) antibody thus serves as a critical reagent for exploring non-catalytic regulatory mechanisms in cell biology and disease pathogenesis.