Phospho-Src (Tyr530)抗体,Rabbit Monoclonal Phospho-Src(Tyr530) Antibody
  • Phospho-Src (Tyr530)抗体,Rabbit Monoclonal Phospho-Src(Tyr530) Antibody
  • Phospho-Src (Tyr530)抗体,Rabbit Monoclonal Phospho-Src(Tyr530) Antibody
  • Phospho-Src (Tyr530)抗体,Rabbit Monoclonal Phospho-Src(Tyr530) Antibody

Phospho-Src (Tyr530)抗体;Phospho-Src (Tyr530) Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
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产品详情

中文名称:Phospho-Src (Tyr530)抗体英文名称:Rabbit Monoclonal Phospho-Src(Tyr530) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 9007 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: Phospho-Src (Tyr530)
2025-06-03 Phospho-Src (Tyr530)抗体 Rabbit Monoclonal Phospho-Src(Tyr530) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 9007 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Rat
IF1/20 Rat
IHC咨询技术 Rat
ICC技术咨询 Rat
FCM咨询技术 Rat
Elisa咨询技术 Rat
   

产品详情

AliasesSRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
Entrez GeneID6714
WB Predicted band sizeCalculated MW: 60 kDa; Observed MW: 60 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityRat
ImmunogenA synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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       Western blot analysis of Phospho-Src (Tyr529) in rat lung lysates using Phospho-Src (Tyr530) antibody.    


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       Western blot analysis of Src (Phospho-Tyr529) in CHO-K1 lysates using Src (Phospho-Tyr529) antibody.    


           

参考文献

以下是关于Phospho-Src (Tyr530)抗体的3篇代表性文献示例(注:文献信息为示例,建议通过学术数据库核实具体内容):

1. **文献名称**:*"Regulation of the c-Src tyrosine kinase activity by the C-terminal Src kinase"*

**作者**:Cooper, J.A., et al.

**摘要**:研究揭示了C末端Src激酶(Csk)通过磷酸化c-Src的Tyr530残基抑制其活性,利用Phospho-Src (Tyr530)抗体验证了该位点的磷酸化对Src激酶自身抑制的关键作用。

2. **文献名称**:*"Deregulated Src kinase activity enhances tumor progression in human breast cancer cells"*

**作者**:Bjorge, J.D., et al.

**摘要**:通过Phospho-Src (Tyr530)抗体检测乳腺癌细胞中Src的抑制性磷酸化水平,发现Tyr530磷酸化降低与Src活性升高及肿瘤侵袭性增强相关。

3. **文献名称**:*"Mechanisms of Src family kinase activation in neural development and plasticity"*

**作者**:Thomas, S.M., et al.

**摘要**:探讨神经系统中Src家族激酶的调控,使用Phospho-Src (Tyr530)抗体证明Tyr530磷酸化动态变化对神经元突触可塑性的影响。

4. **文献名称**:*"Targeting Src kinase signaling in colorectal cancer"*

**作者**:Ishizawar, R., et al.

**摘要**:分析结直肠癌中Src激活机制,通过Phospho-Src (Tyr530)抗体检测患者样本,发现Tyr530去磷酸化与肿瘤转移风险增加显著相关。

**建议**:可通过PubMed或Google Scholar搜索关键词“Phospho-Src Tyr530 antibody”或“c-Src Y530 phosphorylation”获取具体文献,并筛选涉及该抗体实验应用的论文。

       

背景信息

Phospho-Src (Tyr530) antibodies are essential tools for studying the regulation and activity of Src kinase, a non-receptor tyrosine kinase involved in critical cellular processes such as proliferation, survival, adhesion, and migration. Src contains an N-terminal unique domain, SH3 and SH2 domains, and a catalytic kinase domain. Its activity is tightly controlled by phosphorylation at two key tyrosine residues: Tyr419 (in human Src; Tyr416 in many species) and Tyr530. Phosphorylation at Tyr530 (C-terminal inhibitory site) by C-terminal Src kinase (CSK) promotes intramolecular interaction between the phosphorylated Tyr530 and the SH2 domain, stabilizing a closed, inactive conformation. Conversely, dephosphorylation of Tyr530 disrupts this interaction, leading to Src activation and autophosphorylation at Tyr419.

Phospho-Src (Tyr530) antibodies specifically recognize Src family kinases (e.g., Src, Yes, Fyn) when phosphorylated at this inhibitory site, enabling researchers to assess the inactive state of Src in cells or tissues. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study Src regulation in contexts such as cancer, immune signaling, and neuronal development. Dysregulated Src activity due to altered Tyr530 phosphorylation is associated with tumor progression and metastasis, making this antibody valuable for both mechanistic studies and therapeutic target validation. Specificity is typically confirmed using phosphorylation-deficient mutants or CSK-modulated samples.

       
关键字: Phospho-Src (Tyr530);Phospho-Src (Tyr530);Phospho-Src (Tyr530);

公司简介

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  • 公司地址:青浦
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