Phospho-Histone H3 (Thr3)抗体;Phospho-Histone H3 (Thr3) Antibody—艾普蒂 新品

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     Gel: 8%SDS-PAGE, Lysate: 20 μg, Lane: Human fetal muscle tissue lysate, Primary antibody: P08895(TPSB2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08895(TPSB2 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P08895(TPSB2 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

Phospho-Histone H3 (Thr3) antibodies are essential tools for studying mitotic events and chromatin dynamics during the cell cycle. Histone H3. a core component of nucleosomes, undergoes post-translational modifications, including phosphorylation, to regulate chromatin structure and gene expression. Phosphorylation at threonine 3 (Thr3) is a conserved epigenetic marker linked to chromosome condensation and segregation during mitosis. This modification is mediated by the kinase Haspin (GSG2), which specifically targets Thr3 during late G2 and early M phases. Unlike the more widely studied phosphorylation at Ser10 or Ser28. Thr3 phosphorylation facilitates the recruitment of Aurora B kinase to centromeres, a critical step for ensuring proper sister chromatid cohesion and error correction during mitosis.

Antibodies against Phospho-Histone H3 (Thr3) are widely used in cell biology to identify mitotic cells, quantify mitotic indices, and investigate defects in chromosome dynamics. They are applied in techniques like immunofluorescence, flow cytometry, and Western blotting to visualize spatial and temporal phosphorylation patterns in cells or tissues. In cancer research, these antibodies help assess tumor proliferation rates, as elevated Thr3 phosphorylation correlates with aggressive malignancies. Additionally, they serve as biomarkers for evaluating the efficacy of anti-mitotic drugs targeting Haspin or Aurora kinases. However, interpretation requires caution, as cross-reactivity with other phosphorylated residues or nonspecific binding may occur. Overall, these antibodies provide critical insights into cell cycle regulation, genomic stability, and disease mechanisms.