The Phospho-MSK1 (Ser376) antibody is a specialized tool used to detect the activated form of mitogen- and stress-activated protein kinase 1 (MSK1), specifically phosphorylated at serine residue 376. MSK1. a serine/threonine kinase downstream of the MAPK (mitogen-activated protein kinase) signaling pathway, plays a critical role in regulating gene expression by phosphorylating transcription factors such as CREB and NF-κB. Its activation involves dual phosphorylation: first by ERK or p38 MAPK at Thr581/Thr700. and subsequently by autophosphorylation at Ser376 within the C-terminal regulatory domain. The phosphorylation at Ser376 is essential for MSK1's full enzymatic activity and nuclear translocation, linking extracellular stimuli (e.g., stress, growth factors) to transcriptional responses.
This antibody is widely used in research to study MSK1 activation dynamics in cellular processes like inflammation, proliferation, and apoptosis. It enables the detection of phosphorylated MSK1 via techniques such as Western blotting, immunofluorescence, or immunoprecipitation, providing insights into pathway activity in disease models (e.g., cancer, immune disorders). Validation typically includes testing in knockout cells or phosphatase-treated lysates to confirm specificity. Researchers utilize it to explore MSK1's role in signaling crosstalk, drug response, or as a potential therapeutic target.