Phospho-BRAF (Thr401)抗体;Phospho-BRAF (Thr401) Antibody—艾普蒂

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     Western blot analysis of Phospho-B Raf (T401) in (1) PC-12 lysates; (2) PC-12 lysates treated with TPA using Phospho-BRAF (Thr401) antibody.    

  
  

The Phospho-BRAF (Thr401) antibody is a specialized tool used to detect the phosphorylation status of BRAF, a critical serine/threonine kinase in the MAPK/ERK signaling pathway. BRAF plays a central role in regulating cell growth, differentiation, and survival. Phosphorylation at specific residues, such as Thr401. modulates its kinase activity and interactions with downstream effectors like MEK and ERK. Thr401 phosphorylation is implicated in BRAF’s activation or regulatory processes, though its exact functional significance remains under investigation, as BRAF activation is primarily associated with phosphorylation at other residues (e.g., Ser446/447/448 in the activation loop).

This antibody is particularly valuable in cancer research, as aberrant BRAF signaling—often due to mutations like V600E—drives tumorigenesis in melanoma, colorectal cancer, and other malignancies. Detecting Thr401 phosphorylation helps researchers study post-translational modifications that fine-tune BRAF activity or its crosstalk with upstream regulators (e.g., RAS) or inhibitory proteins. Validated applications include Western blotting, immunofluorescence, and immunohistochemistry, often using cell lysates or tissue samples treated with pathway agonists/inhibitors to assess dynamic phosphorylation events. Specificity is typically confirmed using phosphorylation-blocking peptides or BRAF-deficient models.

Understanding Thr401 phosphorylation contributes to unraveling BRAF’s complex regulation and may inform therapeutic strategies targeting hyperactive MAPK signaling in cancer. However, its role may vary across cellular contexts, necessitating careful experimental interpretation.