The Phospho-SIRT1 (Ser47) antibody is a specialized tool used to detect the phosphorylation of SIRT1 (Sirtuin 1) at serine residue 47. a post-translational modification implicated in regulating SIRT1 activity and function. SIRT1. a NAD⁺-dependent class III histone deacetylase, plays critical roles in aging, metabolism, stress response, and cellular homeostasis by deacetylating target proteins, including histones, transcription factors (e.g., p53. FOXOs), and metabolic enzymes. Phosphorylation at Ser47 has been suggested to modulate SIRT1’s subcellular localization, stability, or interaction with substrates, though its precise mechanistic role remains under investigation. Studies indicate that this modification may influence SIRT1’s involvement in pathways such as autophagy, DNA repair, and insulin signaling. The antibody is widely employed in research to explore SIRT1 regulation in conditions like cancer, metabolic disorders, and neurodegenerative diseases. Its development and validation (e.g., via knockout controls or phosphatase treatment) ensure specificity for phosphorylated SIRT1. enabling detection in techniques like Western blotting, immunohistochemistry, or immunofluorescence. Understanding Ser47 phosphorylation dynamics could provide insights into therapeutic strategies targeting SIRT1-related pathways.