Phospho-Smad3 (Ser425)抗体;Phospho-Smad3 (Ser425) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-Smad3 (Ser425) in various lysates using Phospho-Smad3 (Ser425) antibody.    

  
  

Phospho-Smad3 (Ser425) antibodies are essential tools for studying the TGF-β (transforming growth factor-beta) signaling pathway, which regulates diverse cellular processes, including proliferation, differentiation, and apoptosis. Smad3. a member of the receptor-regulated Smad (R-Smad) family, is phosphorylated at specific C-terminal serine residues (e.g., Ser423/425) upon TGF-β receptor activation. This phosphorylation triggers Smad3's dissociation from the receptor, enabling its association with Smad4 and subsequent nuclear translocation to regulate target gene expression. The Phospho-Smad3 (Ser425) antibody specifically detects Smad3 when phosphorylated at Ser425. a key modification linked to its activation and transcriptional activity.

These antibodies are widely used in biomedical research to investigate TGF-β pathway dynamics in diseases such as cancer, fibrosis, and autoimmune disorders. For example, they help assess Smad3 activation status in cell lines, tissues, or animal models treated with TGF-β ligands or inhibitors. Validation often involves Western blotting, immunofluorescence, or immunohistochemistry, with controls like TGF-β-stimulated versus unstimulated samples or kinase inhibitors to confirm specificity. Aberrant Smad3 phosphorylation is associated with pathological conditions, making this antibody valuable for mechanistic studies and therapeutic targeting. Researchers also utilize it to explore crosstalk between TGF-β signaling and other pathways, such as MAPK or PI3K/AKT, in disease contexts.