Phospho-CAD (Thr456)抗体;Phospho-CAD (Thr456) Antibody—艾普蒂 新品

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     Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-CAD (Thr456) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.    

  
  

Phospho-CAD (Thr456) antibody is a specialized tool used to detect the phosphorylated form of CAD (carbamoyl-phosphate synthetase 2. aspartate transcarbamylase, and dihydroorotase), a multifunctional enzyme critical for de novo pyrimidine biosynthesis. CAD catalyzes the first three steps in this pathway, generating uridine monophosphate (UMP), a precursor for DNA and RNA synthesis. Its activity is tightly regulated by phosphorylation, particularly in response to growth signals. The Thr456 residue, located within CAD’s regulatory domain, is a key phosphorylation site targeted by kinases such as Akt (Protein Kinase B) or RSK (ribosomal S6 kinase). Phosphorylation at Thr456 relieves CAD’s autoinhibition, activating pyrimidine synthesis to support cell proliferation during the G1/S phase transition.

This antibody specifically recognizes CAD when phosphorylated at Thr456. enabling researchers to study its activation status in cellular contexts. It is widely used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to explore CAD regulation in cancer, developmental biology, or metabolic studies. For example, elevated phospho-CAD (Thr456) levels may indicate hyperactivation of growth signaling pathways (e.g., PI3K/Akt/mTOR) in tumors, linking pyrimidine metabolism to oncogenesis. Validation often includes testing in cells treated with growth factors, kinase inhibitors, or phosphatase inhibitors, as well as using phosphorylation-deficient mutants to confirm specificity. Its application helps unravel connections between metabolic reprogramming, cell cycle progression, and disease mechanisms.