Phospho-CDC25C (Ser198)抗体;Phospho-CDC25C (Ser198) Antibody—艾普蒂 新品

+ +

•  

   

     Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-CDC25C (Ser198) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.    

  
  

The Phospho-CDC25C (Ser198) antibody is a specialized tool used to study the post-translational modification of CDC25C, a critical phosphatase in cell cycle regulation. CDC25C plays a key role in the G2/M transition by dephosphorylating and activating cyclin-dependent kinase 1 (CDK1), which drives entry into mitosis. Phosphorylation at Ser198 is a regulatory mechanism linked to the DNA damage response. Upon DNA damage, the ATR/CHK1 kinase pathway phosphorylates CDC25C at Ser198. promoting its binding to 14-3-3 proteins. This interaction sequesters CDC25C in the cytoplasm, preventing CDK1 activation and inducing cell cycle arrest, thereby allowing time for DNA repair.

The Phospho-CDC25C (Ser198) antibody specifically detects this phosphorylation event, enabling researchers to study checkpoint activation, cell cycle dynamics, and DNA damage signaling in experimental models. It is widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to assess CDC25C regulation in contexts such as cancer biology, genotoxic stress responses, and drug development. This antibody is particularly valuable for investigating how dysregulation of CDC25C phosphorylation contributes to genomic instability or chemoresistance, offering insights into therapeutic strategies targeting cell cycle checkpoints. Its specificity for the phosphorylated form ensures accurate detection of the active regulatory state of CDC25C under physiological or stress conditions.