产品概述
Topoisomerase I Rabbit Monoclonal Antibody
Rabbit Monoclonal antibody
Rabbit
WB,IHC-F,IHC-P,ICC/IF
Human,Mouse,Rat
产品性能
Unconjugated
Unmodified
IgG
Monoclonal Antibody
Liquid
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Affinity Purification
免疫原
TOP1
TOP1; DNA topoisomerase 1; DNA topoisomerase I
7150
P11387
产品应用
WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:50-1:200
Calculated MW: 91 kDa; Observed MW: 91 kDa
研究背景
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.
研究领域
Epigenetics and Nuclear Signaling
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