产品概述
| 产品名称（Product Name） | Dnmt3b Rabbit Polyclonal Antibody |
| 描述（Description） | Rabbit Polyclonal Antibody |
| 宿主（Host） | Rabbit |
| 应用（Application） | WB,IHC-P,IF-P,IF-F,ICC/IF,ELISA |
| 种属反应性（Reactivity） | Human,Chicken |
产品性能
| 偶联物（Conjugation） | Unconjugated |
| 修饰（Modification） | Unmodified |
| 同种型（Isotype） | IgG |
| 克隆（Clonality） | Polyclonal |
| 形式（Form） | Liquid |
| 存放说明（Storage） | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 储存溶液（Buffer） | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N. |
| 纯化方式（Purification） | Affinity purification |
免疫原
| 基因名（Gene Name） | DNMT3B |
| 别名（Alternative Names） | DNMT3B; DNA (cytosine-5)-methyltransferase 3B; Dnmt3b; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; M.HsaIIIB |
| 基因ID（Gene ID） | 1789 |
| 蛋白ID（SwissProt ID） | Q9UBC3 |
产品应用
| 稀释比（Dilution Ratio） | WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/ICC/IF 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications. |
| 蛋白分子量（Molecular Weight） | 96kDa |
研究背景
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011],catalytic activity:S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.,disease:Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome (ICF) [MIM:242860]. ICF is a rare autosomal recessive disorder characterized by a variable immunodeficiency, mild facial anomalies, and centromeric heterochromatin instability involving chromosomes 1, 9, and 16. ICF is biochemically characterized by hypomethylation of CpG sites in some regions of heterochromatin.,function:Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.,online information:DNMT3B mutation db,PTM:Sumoylated.,similarity:Belongs to the C5-methyltransferase family.,similarity:Contains 1 ADD-type zinc finger.,similarity:Contains 1 PWWP domain.,subunit:Interacts with SUV39H1 (By similarity). Interacts with SETDB1, UBL1 and UBE2I9. Interacts with DNMT1 and DNMT3A. Interacts with the PRC2/EED-EZH2 complex.,tissue specificity:Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.,
研究领域
Cysteine and methionine metabolism;
关键字： DNMT3B;Dnmt3b;Rabbit;Polyclonal;Antibody;一抗
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