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Phospho-PKC zeta 抗体

品牌：MedChemExpress (MCE)

货号：HY-P80280

存储条件：储存于 -20°C 下可保存 1 年。避免反复冻融。

运输条件：使用蓝冰运送。

产品活性：Phospho-PKC zeta (T560) Antibody 是一个非偶联、分子量约 68 kDa、兔源、抗磷酸化 PKC zeta(T560) 单克隆抗体。它可用于人、小鼠背景下 WB、IHC-P 实验，且不带标记。

宿主：兔

反应物种：Human, Mouse

推荐稀释比例：WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200 Western blot analysis of extracts from Hela(lane 2(20μg) ) and Hela(lane 3(20μg), Calyculin A treated (HY-18983, 50nM, 3hrs) as indicated, using Phospho-PKC zeta (T560) (HY-P80280) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80280, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

标签：Free

克隆性：Recombinant, Monoclonal

偶联：Non-conjugated

修饰：Phosphorylated

亚细胞定位：Cell junction, Cytoplasm, Endosome, Membrane.

研究领域：Signal Transduction

蛋白数据库：SwissProt: Q05513 Human;SwissProt: Q02956 Mouse;SwissProt: P09217 Rat

同型：IgG

敏感性：Endogenous

详情参考：www.medchemexpress.cn/antibody/phospho-pkc-zeta-t560-rabbit-mab.html