DNA Damage Antibody Sampler Kit

品牌:CST，产品名称：DNA Damage Antibody Sampler Kit #9947
英文名称：DNA Damage Antibody Sampler Kit
Product name：DNA Damage Antibody Sampler Kit #9947
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.
Background： Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). p53 is phosphorylated by ATM, ATR and DNA-PK at Ser15. This phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,5). Chk1 and Chk2, downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint control, embryonic development and tumor suppression (6). Chk1 is phosphorylated at Ser280 and Ser296 following DNA damage. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues, including Thr68, each followed by glutamine (SQ or TQ motif). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (7-9). The breast cancer susceptibility proteins BRCA1 and BRCA2 are frequently mutated in cases of hereditary breast and ovarian cancers and have roles in multiple processes related to DNA damage, repair, cell cycle progression, transcription, ubiquitination and apoptosis. Numerous DNA-damage induced phosphorylation sites on BRCA1 have been identified, including serine 1524, and kinases activated in a cell cycle-dependent manner, including Aurora A and CDK2, can also phosphorylate BRCA1. IR, DNA and radiometric-induced DNA damage also results in rapid phosphorylation of the histone H2A family member H2A.X at Ser139 by ATM (10,11). Within minutes following DNA damage, Ser139-phosphorylated H2A.X localizes to sites of DNA damage at subnuclear foci (12).

Specificity / Sensitivity:

All antibodies in the DNA Damage Antibody Sampler Kit recognize their targets proteins only when modified at the indicated site.

Protocols

• 2197:

  Western Blotting

  ,

  Immunoprecipitation (Magnetic)

  ,

  Immunohistochemistry (Paraffin)

  ,

  Flow

• 2348:

  Western Blotting

  ,

  Immunofluorescence

  ,

  Flow

• 2853:

  Western Blotting

• 5883:

  Western Blotting

• 7074:

  Western Blotting

• 7076:

  Western Blotting

• 9009:

  Western Blotting

• 9286:

  Western Blotting

  ,

  Immunofluorescence

  ,

  Flow

• 9718:

  Western Blotting

  ,

  Immunohistochemistry (Paraffin)

  ,

  Immunofluorescence

  ,

  Flow

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
Phospho-ATR (Ser428) Antibody 2853	20 µl	WB	H M R Mk	300	Rabbit
Phospho-BRCA1 (Ser1524) Antibody 9009	20 µl	WB	H	220	Rabbit
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb 2197	20 µl	WB IP IHC F	H	62	Rabbit IgG
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb 2348	20 µl	WB IF F	H M R Mk	56	Rabbit IgG
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb 9718	20 µl	WB IHC IF F	H M R Mk	15	Rabbit IgG
Phospho-p53 (Ser15) (16G8) Mouse mAb 9286	20 µl	WB IF F	H	53	Mouse IgG1
Phospho-ATM (Ser1981) (D6H9) Rabbit mAb 5883	20 µl	WB	H	350	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB	Goat
Anti-mouse IgG, HRP-linked Antibody 7076	100 µl	WB	Horse

Product Description:

This kit provides an economical means to analyze major signaling checkpoints in response to DNA damage. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.