HEMOGLOBIN Chemische Eigenschaften,Einsatz,Produktion Methoden
Beschreibung
Haemoglobin, the oxygen-transport protein in the red blood cells, is a tetramer and each of the four chains
contains a haeme group. It is interesting to note that the four haeme groups in haemoglobin do not operate
independently. The release (and binding) of oxygen is a cooperative process, which means that the loss
(uptake) of the first oxygen molecule triggers the release of the remaining three.
The current model for oxygen binding in haemoglobin and myoglobin can be explained in the following
way. The deoxy form contains a high-spin Fe(II) centre, which, because of its size, does not form a plane with
its four nitrogen donor atoms. Instead, it is located slightly above the plane, drawn towards the His residue.
Once oxygen enters trans to the His residue, the iron centre is oxidised to a low-spin Fe
3+ centre and O
2 is
reduced to [O
2]
-. Both species contain an unpaired electron. The low-spin Fe
3+ moves into the plane and
pulls the His residue down. This affects the remaining protein chain and triggers the uptake/release of oxygen
in the other three haeme groups.
Verwenden
Medicine, usually called hemoglobin.
Definition
The respiratory protein of the red blood cells, it transfers oxygen from the lungs to the tissues and carbon dioxide from the tissues to the lungs. Its affinity for carbon monoxide is >200 times that for oxygen. Hemoglobin is a conjugated protein of molec
Allgemeine Beschreibung
Native hemoglobin from bovine erythrocytes. A major oxygen-transporting component of red blood cells that is also nitric oxide scavenger. Blocks carbachol-stimulated cGMP production. This preparation contains primarily Ferric-hemoglobin and must be reduced to the ferrous form to bind molecular oxygen.
Note: this preparation contains primarily ferric-hemoglobin and must be reduced to the ferrous form to bind molecular oxygen.
läuterung methode
Purify it from blood using CM-32 cellulose column chromatography. [Matsukawa et al. J Am Chem Soc 107 1108 1985.] For the purification of the and chains see Hill et al. Biochemical Preparations 10 55 1963. Histones (from S4A mouse lymphoma). The purification of histones uses a macroprocess column, heptafluorobutyric acid as solubilising and ion-pairing agent and an acetonitrile gradient. [McCroskey et al. Anal Biochem 163 427 1987.]
HEMOGLOBIN Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
