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2-Methyl-4-isothiazolin-3-one

2-Methyl-4-isothiazolin-3-one Structure
2-Methyl-4-isothiazolin-3-one structure
CAS No.
2682-20-4
Chemical Name:
2-Methyl-4-isothiazolin-3-one
Synonyms
Methylisothiazolinone;2-METHYL-3(2H)-ISOTHIAZOLONE;2-METHYL-4-ISOTHIAZOLIN-3-ONE;2-Methylisothiazol-3(2H)-one;2-methyl-2H-isothiazol-3-one;Casson preservatives;2-Methyl-4-isothiazolin-3-on;2-METHYL-4-ISOTHIAZOLINE-3-ONE;3(2H)-Isothiazolone, 2-methyl-;METHYLISOTHIAZOLONE
CBNumber:
CB0193926
Molecular Formula:
C4H5NOS
Molecular Weight:
115.15
MDL Number:
MFCD00013254
MOL File:
2682-20-4.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-06-05 18:44:30
Product description Number Pack Size Price
2-Methyl-4-isothiazolin-3-one 95% 725765 5g $114
2-Methyl-4-isothiazolin-3-one 95% 725765 25g $452
Methylisothiazolinone ≥98% 28136 1mL $31
Methylisothiazolinone ≥98% 28136 5mL $85
Methylisothiazolinone ≥98% 28136 10mL $139
More product size
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2-Methyl-4-isothiazolin-3-one Properties

Melting point 254-256 °C(lit.)
Boiling point bp0.03 93°
Density 1.25 (14% aq.)
vapor pressure <0.1 mm Hg ( 25 °C)
storage temp. 2-8°C
solubility Chloroform, Ethyl Acetate
form Solid
pka -2.03±0.20(Predicted)
color Yellow
Water Solubility 489g/L at 20℃
BRN 606203
Cosmetics Ingredients Functions PRESERVATIVE
Cosmetic Ingredient Review (CIR) 2-Methyl-4-isothiazolin-3-one (2682-20-4)
InChI 1S/C4H5NOS/c1-5-4(6)2-3-7-5/h2-3H,1H3
InChIKey BEGLCMHJXHIJLR-UHFFFAOYSA-N
SMILES CN1SC=CC1=O
LogP -0.486 at 20℃
CAS DataBase Reference 2682-20-4(CAS DataBase Reference)
Indirect Additives used in Food Contact Substances 2-METHYL-4-ISOTHIAZOLIN-3-ONE
FDA 21 CFR 175.105(Adhesives); 175.300(Resinous and polymeric coatings); 175.320(Resinous and polymeric coatings for polyolefin films);
176.170(Components of paper and paperboard in contact with aqueous and fatty foods)
EWG's Food Scores 4-7
FDA UNII 229D0E1QFA
NIST Chemistry Reference 3(2H)-isothiazolone, 2-methyl-(2682-20-4)
EPA Substance Registry System 2-Methyl-3(2H)-isothiazolone (2682-20-4)
UNSPSC Code 41116107
NACRES NA.24

SAFETY

Risk and Safety Statements

Symbol(GHS)  Corrosion (GHS05)Skull and Crossbones (GHS06)Environment (GHS09)
GHS05,GHS06,GHS09
Signal word  Danger
Hazard statements  H301+H311-H314-H317-H330-H410
Precautionary statements  P260-P273-P280-P303+P361+P353-P304+P340+P310-P305+P351+P338
PPE dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Hazard Codes  N,C,T
Risk Statements  50/53-50-43-34-20/21/22-37-23-22
Safety Statements  60-61-36/37/39-26-24-45
RIDADR  UN 3077 9/PG 3
WGK Germany  2
RTECS  FE1250000
TSCA  TSCA listed
HazardClass  8
PackingGroup  III
HS Code  29349990
Storage Class 6.1A - Combustible acute toxic Cat. 1 and 2
very toxic hazardous materials
Hazard Classifications Acute Tox. 2 Inhalation
Acute Tox. 3 Dermal
Acute Tox. 3 Oral
Aquatic Acute 1
Aquatic Chronic 1
Eye Dam. 1
Skin Corr. 1B
Skin Sens. 1A
Hazardous Substances Data 2682-20-4(Hazardous Substances Data)
REACH Registrations Active

2-Methyl-4-isothiazolin-3-one price More Price(66)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 725765 2-Methyl-4-isothiazolin-3-one 95% 2682-20-4 5g $114 2026-04-30 Buy
Sigma-Aldrich 725765 2-Methyl-4-isothiazolin-3-one 95% 2682-20-4 25g $452 2026-04-30 Buy
Cayman Chemical 28136 Methylisothiazolinone ≥98% 2682-20-4 1mL $31 2026-04-30 Buy
Cayman Chemical 28136 Methylisothiazolinone ≥98% 2682-20-4 5mL $85 2026-04-30 Buy
Cayman Chemical 28136 Methylisothiazolinone ≥98% 2682-20-4 10mL $139 2026-04-30 Buy
Product number Packaging Price Buy
725765 5g $114 Buy
725765 25g $452 Buy
28136 1mL $31 Buy
28136 5mL $85 Buy
28136 10mL $139 Buy

2-Methyl-4-isothiazolin-3-one Chemical Properties,Uses,Production

Description

Methylisothiazolinone (also called 2-methyl-4-isothiazolin-3-one), is a powerful synthetic biocide and preservative within the group of  isothiazolinones.
Methylisothiazolinone is used to control slime-forming bacteria, fungi, and algae in pulp/paper mills, cooling water systems, oil field operations, industrial process waters, and air washer systems. And it is incorporated into adhesives, coatings, fuels, metal working fluids, resin emulsions, paints, and various other specialty industrial products as a preservative. It is also used to control the growth of mold, mildew, and sapstain on wood products. It is generally recommended for use only in rinse-off and leave-on cosmetic products (maximum concentration of 100 ppm) as preservative such as shampoo, conditioner, hair color, body wash, lotion, sunscreen, mascara, shaving cream, baby lotion, baby shampoo, hairspray, makeup remover, liquid soaps, and detergents. Nevertheless, methylisothiazolinone is allergenic. It is reported that methylisothiazolinone in rinse-off products causes allergic contact dermatitis.

Uses

2-Methyl-4-isothiazolin-3-one, or MIT as it is sometimes known, is a preservative used in cosmetics and beauty products. It is a powerful biocide, or &ldquo;chemical substance capable of killing living organisms, usually in a selective way.&rdquo;Biocides are a general term that includes antimicrobial, germicide, antibiotic, and antifungal. Ultimately, Methylisothiazolinone is used to prevent a wide variety of bacteria and fungi from growing in cosmetics and beauty products, most often in shampoo. It is only approved for use in rinse-off formulas and at low concentrations.

Safety

Methylisothiazolinone (MIT) is a heterocyclic organic compound used as a preservative in cosmetics and personal care products in concentrations up to 0.01%. MIT is a colorless, clear liquid with a mild odor that is completely soluble in water; mostly soluble in acetonitrile, methanol, and hexane; and slightly soluble in xylene. Consistent with its solubility, dermal penetration is low. The Cosmetic Ingredient Review Expert Panel noted the in vitro evidence of neurotoxicity but concluded that the absence of any neurotoxicity findings in the many in vivo studies, including subchronic, chronic, and reproductive and developmental animal studies, suggests that MIT would not be neurotoxic as used in cosmetics. Although recognizing that MIT was a sensitizer in both animal and human studies, the panel concluded that there is a threshold dose response and that cosmetic products formulated to contain concentrations of MIT at 100 ppm (0.0 1%) or less would not be expected to pose a sensitization risk. Accordingly, MIT may be safely used as a preservative in cosmetics up to that concentration.

References

[1] http://www.safecosmetics.org/get-the-facts/chemicals-of-concern/methylisothiazolinone/
[2] K. Yazar, M. D. Lundov, A. Faurschou, M. Matura, A. Boman, J. D. Johansen, C. Lidén (2015) Methylisothiazolinone in rinse-off products causes allergic contact dermatitis: a repeated open-application study, 173, 115-122
[3] https://www3.epa.gov/pesticides/chem_search/reg_actions/reregistration/fs_G-58_1-Oct-98.pdf

Description

2-Methyl-4-isothiazolin-3-one (MI) is an isothiazolinone-derived biocide used for controlling microbial growth in industrial and household products, often in a mixture with 5-chloro-2-methyl-3-isothiazolone (MCI). MI is active against Gram-positive and Gram-negative bacteria, fungi, and yeast with MIC values of 0.0045, 0.0015, >0.01, and 0.0065% (w/w) for S. aureus, P. aeruginosa, A. niger, and C. albicans, respectively, when used alone. MIC values are 7 to 200-fold lower when MI is used in combination with MCI. MI decreases neurite outgrowth of rat cortical neurons when used at concentrations of 0.1-3 μM and inhibits Src family kinases in cell-free assays. MI, alone and as a mixture with MCI, can elicit contact sensitization.

Chemical Properties

2-Methyl-4-isothiazolin-3-one (MIT) is a colorless,clear liquid with amild odor that is completely soluble in water; mostly soluble in acetonitrile, methanol, and hexane; and slightly soluble in xylene. MIT is a heterocyclic organic compound used as a preservative in cosmetics and personal care products in concentrations up to 0.01%.

Uses

2-Methyl-4-isothiazolin-3-one (methylisothiazolinone) is a isothiazolinone based biocide and preservative used in personal care products. 2-Methyl-4-isothiazolin-3-one is also used for controlling microbial growth in water-c ontaining solution.

Uses

Methylisothiazolinone is a preservative compound widely used in cosmetics. It is a contact allergen and sensitiser. Methylisothiazolinone has recently been identified as a neurotoxin that can damage nerve endings with repeated exposure.

Definition

ChEBI: Methylisothiazolinone is a 1,2-thazole that is 4-isothiazolin-3-one bearing a methyl group on the nitrogen atom. It is a powerful biocide and preservative and is the minor active ingredient in the commercial product Kathon(TM). It has a role as an antifouling biocide, an antimicrobial agent and an antifungal agent.

Preparation

2-Methyl-4-isothiazolin-3-one (MIT)is prepared by cyclization of cis-N-methyl-3-thiocyanoacrylamide:    NCSCH=CHC(O)NHCH3SCH=CHC(O)NCH3+HCN

Hazard

2-Methyl-4-isothiazolin-3-one(MIT)is allergenic and cytotoxic, and this has led to some concern over its use.A report released by the European Scientific Committee on Cosmetic Products and Non-food Products Intended for Consumers (SCCNFP) in 2003 also concluded that insufficient information was available to allow for an adequate risk assessment analysis of MIT.

Contact allergens

MI is generally associated with MCI, in Kathon CG, MCI/MI, and Euxyl K 100. This preservative is currently used in water-based products such as cosmetics, paints, and glues. Skin contact with concentrated solution can cause severe irritant dermatitis.

Side effects

Early on, dermatitis may occur only on part of the exposed skin. Common patterns include: hand dermatitis，perianal dermatitis, perivulval dermatitis, napkin dermatitis, facial dermatitis, eyelid swelling, and scalp dermatitis. Later, more extensive and severe whole-body contact dermatitis may occur in very sensitive people.

Toxics Screening Level

Due to a lack of toxicity information on this particular chemical, the screening level is being established under R232(1)(i) at 0.1 μg/m3 with annual averaging.

References

[1] ELRIKE FRENZEL. Importance of porins for biocide efficacy against Mycobacterium smegmatis.[J]. Applied and Environmental Microbiology, 2011, 77 9: 3068-3073. DOI: 10.1128/aem.02492-10
[2] M. D. LUNDOV. Low-level efficacy of cosmetic preservatives[J]. International Journal of Cosmetic Science, 2011, 33 2: 190-196. DOI: 10.1111/j.1468-2494.2010.00619.x
[3] KAI HE. Methylisothiazolinone, a neurotoxic biocide, disrupts the association of SRC family tyrosine kinases with focal adhesion kinase in developing cortical neurons.[J]. Journal of Pharmacology and Experimental Therapeutics, 2006, 317 3: 1320-1329. DOI: 10.1124/jpet.106.103044
[4] MARIA ANTONIETA RIOS SCHERRER  Vanessa B R. Increasing trend of sensitization to Methylchloroisothiazolinone/methylisothiazolinone (MCI/MI).[J]. Anais brasileiros de dermatologia, 2014, 89 3: 527-528. DOI: 10.1590/abd1806-4841.20142852

2-Methyl-4-isothiazolin-3-one synthesis

999-72-4
2682-20-4
Synthesis of 2-Methyl-4-isothiazolin-3-one from N,N'-Dimethyl-3,3'-dithiodipropionamide

2-Methyl-4-isothiazolin-3-one Preparation Products And Raw materials

Raw materials

Preparation Products

2-Methyl-4-isothiazolin-3-one Suppliers

Global( 547)Suppliers
Supplier Tel Email Country ProdList Advantage
Jinan Qinmu Fine Chemical Co.,Ltd.
+8618660799346 sales@qinmuchem.com China 1008 58
WUXI HONOR SHINE CHEMICAL CO.,LTD
+86-0510-83593312 +8619805123561 sales@honorshinechem.com China 69 58
Suzhou Genemill Biotechnology Co.,Ltd
+86-0512-65917170 +86-15862502491 glm@genemill.com China 39 58
Hebei Chuanghai Biotechnology Co., Ltd 		+8615350571055 Sibel@chuanghaibio.com China 8738 58
Hebei Yanxi Chemical Co., Ltd. 		+8618531123677 faithe@yan-xi.com China 5853 58
Wuhan Fortuna Chemical Co., Ltd 		+86-27-59207850 info@fortunachem.com China 5983 58
Hebei Chuanghai Biotechnology Co,.LTD 		+86-86-13131129325 +8613131129325 sales1@chuanghaibio.com China 5228 58
Hebei Chuanghai Biotechnology Co., Ltd 		+8615531151365 mina@chuanghaibio.com China 18126 58
Henan Fengda Chemical Co., Ltd 		+86-371-86557731 +86-13613820652 info@fdachem.com China 20122 58
Taicang Puyuan Pharmaceutical Co., Ltd. 		+86-51253988058 +86-13962629881 fanny@puyuanchem.cn China 22 58
Supplier Advantage
Jinan Qinmu Fine Chemical Co.,Ltd.
58
WUXI HONOR SHINE CHEMICAL CO.,LTD
58
Suzhou Genemill Biotechnology Co.,Ltd
58
Hebei Chuanghai Biotechnology Co., Ltd 		58
Hebei Yanxi Chemical Co., Ltd. 		58
Wuhan Fortuna Chemical Co., Ltd 		58
Hebei Chuanghai Biotechnology Co,.LTD 		58
Hebei Chuanghai Biotechnology Co., Ltd 		58
Henan Fengda Chemical Co., Ltd 		58
Taicang Puyuan Pharmaceutical Co., Ltd. 		58

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Q:What is 2-Methyl-4-isothiazolin-3-one on MIT?Qing - Mar 31，2026
A:2-Methyl-4-isothiazolin-3-one (abbreviated as MIT) is a highly effective, broad-spectrum biocide and preservative belonging to the isothiazolinone class. By disrupting microbial cell membranes and inhibiting the activity of metabolic enzymes, it powerfully suppresses the growth of bacteria, fungi, and algae. It is frequently used in combination with 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) in a 3:1 ratio, a blend commercially known as "Kathon." MIT is widely utilized in various applications, including cosmetics, shampoos, lotions, paints, water treatment systems, and industrial circulating water. Chinese regulations stipulate a maximum permissible concentration of 0.01% (100 ppm) for its use in cosmetic products. However, MIT is a known sensitizer and constitutes one of the primary triggers for contact dermatitis; at high concentrations, it can cause skin and eye irritation, while prolonged exposure may potentially lead to neurotoxicity and allergic reactions. Consequently, its use in leave-on cosmetic products requires particular caution.

View Lastest Price from 2-Methyl-4-isothiazolin-3-one manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
2-Methyl-4-Isothiazolin-3-one pictures 2026-06-07 2-Methyl-4-Isothiazolin-3-one
2682-20-4
 0.99 RongNa Biotechnology Co.,Ltd
Methylisothiazolinone pictures 2026-06-05 Methylisothiazolinone
2682-20-4
 1KG 98%min 30tons/month WUHAN FORTUNA CHEMICAL CO., LTD
MIT-50 pictures 2026-05-28 MIT-50
2682-20-4
 25Kg/Drum 50% 3000tons WUXI HONOR SHINE CHEMICAL CO.,LTD
  • MIT-50 pictures
  • MIT-50
    2682-20-4
  • 50%
  • WUXI HONOR SHINE CHEMICAL CO.,LTD

2-Methyl-4-isothiazolin-3-one Spectrum

2-Methyl-4-isothiazolin-3-one(2682-20-4)1HNMR

2682-20-4(2-Methyl-4-isothiazolin-3-one)Related Search:

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1-(4-CHLOROPHENYL)-3-(3,4-DICHLOROPHENYL)UREA 2-METHYL-4-ISOTHIAZOLIN-3-ONE N-METHYL-3-OXODIHYDRO ISOTHIAZOLE 2-methyl-3(2h)-isothiazolon Isothiazolone,2-methyl- Skycide 100 2-Methyl-4-isothiazolin-3-one (Discontinued, see M314985) 2-Methyl-4-iso Isothiazolone 14% 2-methyl-1,2-thiazol-3-one MIT, 100ppm 2-Methylisothiazolin-3-one 2-METHYL-4-ISOTHIAZOLIN-3-ONE (MI) METHYLISOTHIAZOLINE KATHONCG METHYLISOTHIAZDINONE METHYLISOTHIAZOLIN 2-METHYLISOTHIAZOLINONE METHYLISOTHIAZOLONE 2-methyl-4-isothiazolin-3-one MIT 5-Chloro-2-methyl-4-isothiazolin-3-one/2-Methyl-4-isothiazolin (CMIT/MIT-14) 2-Methyl-3-isothiazolone(MIT) 2-METHYL-4-ISOTHIAZOLIN-3-ONE(MIT) 50% WATER SOLUTION MIT50% 2-Methyl-4-isothiazolin (CMIT/MIT-14) 2- METHYL-4-ISOTHIAZOLINE-3-KETONE 2-METHYL-4-ISOTHIAZOLIN-3-ONE, 90+% 2-Methyl-2,3-dihydroisothiazol-3-one 2-Methylisothiazol-3-one Methyl-3(2H)-isothiazolone 3,4,4''-TRICHLOROCARBANILIDE(TCC) 2-Methyl-2H-isothiazole-3-one 2-Methylisothiazole-3-one 2-Methyl-4-isothiazolin-3-one,2-Methyl-3(2H)-isothiazolone 2-Methyl-4-isothiazolin-3-on 2-Methyl-4-Isothiazolin-3-Ketone 2-Methyl-2,3-dihydro-1,2-thiazol-3-one 5-chloro- Methyl-4-thiazoline-3-ketone(MI) 2-Methyl-4-Isothiazolin-3-Ketone (MIT) Methylisothiazolinone(MIT) MIT-50/ Methylisothiazolinone MIT-10/2-methyl-4-ishothiazolin-3-one Different thiazole moiety Methyl isothiazalinone MIT-20 2-Methyl-4-isothiazolin-3-one, 50% aqueous solution Methylisothiazolinone (2-Methyl-4-isothiazolin-3-one) 2-Methyl-4-Isothiazolin-3-one ( MIT 50% ) / Isothiazolinones 50% 2-methyl 4-isoisothiazolin-3-one Biocide Algicide Isothiazolinones CMIT/MIT CAS 26172-55-4 2682-20-4 NeoCide PC-950 preservative Methylisothiazolone，MIT Methyl Isathiazolinone PC-950 Isothiazolinone methyl chloroisothiazolinone and methyl isothiazolinone (CMIT/MIT) 2-Methyl-4-Isothiazolin-3-Ketone | Methylisothiazolinone Carbidopa Impurity 16 Hydrobromide 2-methyl-2H-isothiazol-3-one (MIT) Methylisothiazolinone, 10 mM in DMSO