ACRIDINE ORANGE

ACRIDINE ORANGE Properties

Melting point	284-287 °C(lit.)
Density	1.001 g/mL at 20 °C
refractive index	n20/D 1.338
storage temp.	-20°C
solubility	Soluble in water, ethanol, dimethyl sulfoxide
form	Powder
pka	-3.2, 10.5(at 25℃)
Colour Index	46005
color	orange to red
Water Solubility	H2O: 0.1%, clear, orange to red
ε(extinction coefficient)	≥14,000 at 227-233nm in water at 0.005g/L ≥35000 at 265-271nm in water at 0.005g/L
λmax	500 nm
BRN	3734978
Major Application	Adhesives; aluminophosphate crystalline materials; detecting clay particles; display device; evaluating fiber surface characteristics; glass matrixes; imaging material; inks; lasers; recording materials; photoresists; textiles; thin films; tracers for hydrology; wiring boards
Biological Applications	Detecting cancer cells,spores,human papilloma virus (HPV),stress biomarkers; treating amyloid associated diseases,lupus,pathogen infections; apoptosis assays; cytotoxicity assays
InChI	1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
InChIKey	VSTHNGLPHBTRMB-UHFFFAOYSA-N
SMILES	Cl[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
CAS DataBase Reference	65-61-2
FDA UNII	YO2175B15I
EPA Substance Registry System	C.I. Basic Orange 14 (65-61-2)
UNSPSC Code	12171500
NACRES	NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS08
Signal word	Warning
Hazard statements	H341
Precautionary statements	P201-P202-P280-P308+P313-P405-P501
PPE	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Safety Statements	22-24/25
WGK Germany	3
RTECS	AR7601000
TSCA	TSCA listed
HS Code	32041300
Storage Class	12 - Non Combustible Liquids
Hazard Classifications	Muta. 2
Toxicity	dnd-mus:ast 20 mmol/L BBACAQ 374,96,74

ACRIDINE ORANGE price More Price(51)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	318337	Acridine Orange hydrochloride hydrate 99% (HPLC)	65-61-2	1g	$185	2026-04-30	Buy
Sigma-Aldrich	318337	Acridine Orange hydrochloride hydrate 99% (HPLC)	65-61-2	5g	$794	2026-04-30	Buy
Usbiological	555185	AOX1 Purifiedbyimmunoaffinitychromatography.		50ul	$509	2026-06-03	Buy
Usbiological	555185	AOX1 Purifiedbyimmunoaffinitychromatography.		100ul	$708	2026-06-03	Buy
Usbiological	520117	AOX1 Purifiedbyimmunoaffinitychromatography.		100ul	$736	2026-06-03	Buy

Product number	Packaging	Price	Buy
318337	1g	$185	Buy
318337	5g	$794	Buy
555185	50ul	$509	Buy
555185	100ul	$708	Buy
520117	100ul	$736	Buy

ACRIDINE ORANGE Chemical Properties,Uses,Production

Chemical Properties

dark orange to brown powder

Uses

Acridine orange is a dye that interacts with DNA and RNA by intercelation or electrostatic attraction. This cationic dye has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Preparation

4,4 ‘-Bis (N, N-dimethyl) aminodiphenylmethane obtained dinitration of 4,4′ – bis (N, N-dimethyl) amino-2 ‘- dinitro-diphenyl methane reduction, and with an acid of heat, so that the diamino compound is cyclized product is oxidized and then cyclized and converted into zinc chloride double salt.

Biological Activity

acridine orange hydrochloride is a cell and organelle membrane permeable fluorescent dye that targets nucleic acids. acridine orange can either be incorporated into double helical nucleic acids (detected by green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (detected by red fluorescence at 640 nm), which renders it the ability to differently stain dna and rna or single-stranded dna in situ. for unfixed cells, acridine orange staining can still be used as a simple, fast approach of obtaining information on cell cycle status and cell ploidy levels from dna staining (green fluorescence), as well as cell transcriptional activity from rna measurements (red fluorescence), in a flow cytofluorometric system. thus, acridine orange may serve as a useful cytochemical stain for cell-cycle and apoptosis studies.1. mcmaster gk, carmichael gg. analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. proceedings of the national academy of sciences of the united states of america, 1977, 74(11): 4835-4838.2. traganos f, darzynkiewicz z, sharpless t, et al. simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. journal of histochemistry and cytochemistry, 1977, 25(1): 46-56.

Safety Profile

Mutation data reported. Whenheated to decomposition it emits very toxic fumes of HCl,Cl, and NOx.

Enzyme inhibitor

This cell-permeable dye (FWfree-base = 265.36 g/mol; CAS 65-61-2), also known as N,N,N',N'-tetramethylacridine-3,6-diamine, is an intercalator that binds tightly to nucleic acids. Binding to DNA is characterized by excitation maximum at 502 nm (cyan) and an emission maximum at 525 nm, producing a green fluorescence. (A 120 μM solution of acridine orange will detect 25-50 ng DNA per band in an agarose or polyacrylamide gel.) Binding to RNA is characterized by an excitation maximum at 460 nm and the emission maximum is located at 650 nm, yielding a reddish-orange fluorescence. Acridine orange also penetrates acidic compartments (e.g., lysosomes and phagolysosomes); upon protonation, the dye emits orange light when excited by blue light.

storage

Room temperature

Properties and Applications

bright yellow orange. Soluble in water and ethanol for orange yellow, with green fluorescent. The strong sulfuric acid in nearly colorless, a green fluorescent, diluted for orange. The dye solution with strong sodium hydroxide solution have yellow precipitation. And C.I. Solvent Orange 15 the same chemical structure (free base).

Standard( Silk )	Light Fastness	Persperation Fastness		Ironing Fastness		Soaping
Standard( Silk )	Light Fastness	Fading	Stain	Fading	Stain	Fading	Stain
ISO	1-2	3	1	5		1	3

ACRIDINE ORANGE Preparation Products And Raw materials

Raw materials

Preparation Products

Acridinium, 3,6-bis(dimethylamino)-10-methyl-, methyl sulfate

ACRIDINE ORANGE Suppliers

Global( 194)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Lianyungang Kebyke Biotechnology Co., Ltd.	18011665355	304508608@qq.com	China	121	58
Amel Pharmatech Corporation	027-888-4366503 13986279625	sales@amelpharmatech.com	China	4826	58
J & K SCIENTIFIC LTD.	18210857532 18210857532	jkinfo@jkchemical.com	China	96815	76
Meryer (Shanghai) Chemical Technology Co., Ltd.	4006356688 18621169109	market03@meryer.com	China	40202	62
3B Pharmachem (Wuhan) International Co.,Ltd.	18930552037	3bsc@sina.com	China	15813	69
Beijing Ouhe Technology Co., Ltd	13552068683 13522913783	2355560935@qq.com	China	11777	60
Adamas Reagent, Ltd.	400-6009262 15618770481	yhx@titansci.com	China	14098	59
Thermo Fisher Scientific	800-810-5118	cnchemical@thermofisher.com	China	17752	75
TOKYO CHEMICAL INDUSTRY CO., LTD.	03-36680489	Sales-JP@TCIchemicals.com	Japan	28364	80
TCI Europe	320-37350700	sales@tcieurope.eu	Europe	23654	75

Supplier	Advantage
Lianyungang Kebyke Biotechnology Co., Ltd.	58
Amel Pharmatech Corporation	58
J & K SCIENTIFIC LTD.	76
Meryer (Shanghai) Chemical Technology Co., Ltd.	62
3B Pharmachem (Wuhan) International Co.,Ltd.	69
Beijing Ouhe Technology Co., Ltd	60
Adamas Reagent, Ltd.	59
Thermo Fisher Scientific	75
TOKYO CHEMICAL INDUSTRY CO., LTD.	80
TCI Europe	75

View Lastest Price from ACRIDINE ORANGE manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2026-09-02	ACRIDINE ORANGE 65-61-2	$5.00	1KG	98%		Henan Fengda Chemical Co., Ltd
2026-08-20	Acridine Orange hydrochloride 65-61-2	$47.00		99.99%	10g	TargetMol Chemicals Inc.
2026-07-16	Acridine Orange hydrochloride 65-61-2	$47.00		99.99%	10g	TargetMol Chemicals Inc.

ACRIDINE ORANGE
65-61-2
$5.00
98%
Henan Fengda Chemical Co., Ltd

Acridine Orange hydrochloride
65-61-2
$47.00
99.99%
TargetMol Chemicals Inc.

Acridine Orange hydrochloride
65-61-2
$47.00
99.99%
TargetMol Chemicals Inc.

ACRIDINE ORANGE Spectrum

ACRIDINE ORANGE(65-61-2)¹HNMR ACRIDINE ORANGE(65-61-2)IR

65-61-2(ACRIDINE ORANGE)Related Search:

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ACRIDINE ORANGE ACRIDINE ORANGE 10-NONYL BROMIDE,NONYL ACRIDINE ORANGE Proflavine hemisulfate Acridine Orange Stain 10-Octadecylacridine orange bromide

3,6-Diaminoacridine hydrochloride ACRIDINE ORANGE-10-DODECYL BROMIDE,3,6-Bis(dimethylamino)-10-dodecylacridinium bromide, Acridine orange-10-dodecyl bromide ACRIDINE ORANGE, 1/2ZNCL Acridine Orange in Acetate Buffer ACRIDINE ORANGE 10-NONYL BROMIDE (NONYL ACRIDINE ORANGE)

DIMETHYL-QUINOLIN-7-YL-AMINE Acridine Orange + DNA Acridine Orange + RNA

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