Acridine Orange Stain
- CAS No.
- 494-38-2
- Chemical Name:
- Acridine Orange Stain
- Synonyms
- N3,N3,N6,N6-TetraMethylacridine-3,6-diaMine;ACRIDINE ORANGE BASE;SOLVENT ORANGE 15;c.i.46005b;EUCHRYSINE;CI NO 46005;C.I.46005:1;c.i.no.46005:1;waxolineorangea;oranzakridinova
- CBNumber:
- CB4378880
- Molecular Formula:
- C17H19N3
- Molecular Weight:
- 265.35
- MDL Number:
- MFCD00005029
- MOL File:
- 494-38-2.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Product description | Number | Pack Size | Price |
| Acridine Orange base Dye content 75% | 235474 | 5g | $369 |
| Acridine Orange base Dye content 75% | 235474 | 25g | $1480 |
| Acridine Orange | 14338 | 5g | $69 |
| Acridine Orange | 14338 | 10g | $115 |
| Acridine Orange | 14338 | 25g | $263 |
| More product size | |||
| Melting point | 165 °C (dec.)(lit.) |
|---|---|
| Boiling point | 469℃ |
| Density | 1.169 |
| refractive index | 1.6080 (estimate) |
| Flash point | 237℃ |
| storage temp. | Sealed in dry,Room Temperature |
| solubility | DMF: 2 mg/ml; DMSO: 20 mg/ml; Ethanol: 0.3 mg/ml; PBS (pH 7.2): 1 mg/ml |
| form | A crystalline solid |
| Colour Index | 46005 |
| pka | 9.97±0.30(Predicted) |
| color | Yellow needles from EtOH |
| λmax | 488 nm |
| ε(extinction coefficient) |
≥14000 at 227-233nm ≥20000 at 289-295nm ≥35000 at 486-492nm ≥40000 at 266-272nm |
| Stability | Light Sensitive |
| Major Application |
diagnostic assay manufacturing hematology histology |
| InChI | 1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3 |
| InChIKey | DPKHZNPWBDQZCN-UHFFFAOYSA-N |
| SMILES | CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C |
| FDA UNII | F30N4O6XVV |
| IARC | 3 (Vol. 16, Sup 7) 1987 |
| UNSPSC Code | 12171500 |
| NACRES | NA.47 |
SAFETY
Risk and Safety Statements
| PPE | Eyeshields, Gloves, type N95 (US) |
|---|---|
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
| RTECS | AR7600000 |
| Storage Class | 11 - Combustible Solids |
| Toxicity | mmo-omi 10 mg/L MIBLAO 49,223,80 |
Acridine Orange Stain price More Price(30)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 235474 | Acridine Orange base Dye content 75% | 494-38-2 | 5g | $369 | 2026-04-30 | Buy |
| Sigma-Aldrich | 235474 | Acridine Orange base Dye content 75% | 494-38-2 | 25g | $1480 | 2026-04-30 | Buy |
| Cayman Chemical | 14338 | Acridine Orange | 494-38-2 | 5g | $69 | 2026-04-30 | Buy |
| Cayman Chemical | 14338 | Acridine Orange | 494-38-2 | 10g | $115 | 2026-04-30 | Buy |
| Cayman Chemical | 14338 | Acridine Orange | 494-38-2 | 25g | $263 | 2026-04-30 | Buy |
Acridine Orange Stain Chemical Properties,Uses,Production
Description
Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination and detection of cellular autophagy. It exhibits excitation/emission spectra of 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively. The ratio of acridine orange emission at 590 to emission at 525 nm can be used to quantify the increase in the number of acidic vesicular organelles observed during cellular autophagy.
Uses
Acridine Orange Stain is a fluorescent nucleic acid binding dye which interacts with both DNA and RNA. When bound to DNA, it emits green fluorescence (Em=525 nm) and when bound to RNA, it emits red fluorescence (Em=~650 nm).
Preparation
4,4′-Bis(N,N-dimethyl)aminodiphenylmethanedinitration, the resultant 4-(4-(Dimethylamino)-3-nitrobenzyl)-N,N-dimethyl-2-nitrobenzenaminereduction, and acid heating will diamino compound cyclization, then will product oxidation and made free base.
Definition
ChEBI: A member of the class of aminoacridines that is acridine carrying two dimethylamino substituents at positions 3 and 6. The hydrochloride salt is the fluorescent dye 'acridine orange', used for cell cycle determination.
Safety Profile
Poison by subcutaneous route.Questionable carcinogen with experimental tumorigenicand carcinogenic data. Mutation data reported. Whenheated to decomposition it emits toxic fumes of NOx.
Properties and Applications
brilliant yellow orange. Soluble in water and ethanol as orange, with green fluorescence. In concentrated sulfuric acid in almost colorless, with green fluorescent, dilution after orange. Dye aqueous solution to join sodium hydroxide have yellow precipitation.
| Standard | Light Fastness | Heat-resistant(℃) | water | Sodium Carbonate(5%) | Hydrochloric acid(5%) | |
| Melting point | Stable | |||||
| ISO | Poor | Poor | ||||
Purification Methods
The double salt with ZnCl2 (6g) is dissolved in water (200mL) and stirred with four successive portions (12g each) of Dowex-50 ion-exchange resin (K+ form) to remove the zinc. The solution is then concentrated in vacuum to 20mL, and 100mL of ethanol is added to precipitate KCl which is removed. Ether (160mL) is added to the solution from which, on chilling, the dye crystallises as its chloride. It is separated by centrifugation, washed with chilled ethanol and ether, and dried under vacuum, before being recrystallised from ethanol (100mL) by adding ether (50mL), and chilling. Yield 1g. [Pal & Schubert J Am Chem Soc 84 4384 1962]. It was recrystallised twice as the free base from ethanol or methanol/water by dropwise addition of NaOH (less than 0.1M). The precipitate was washed with water and dried under vacuum. It was dissolved in CHCl3 and chromatographed on alumina: the main sharp band was collected, concentrated and cooled to -20o. The precipitate was filtered off, dried in air, then dried for 2hours under vacuum at 70o. [Stone & Bradley J Am Chem Soc 83 3627 1961, Blauer & Linschitz J Phys Chem 66 453 1962, Albert J Chem Soc 244 1947, Beilstein 22 III/IV 5490, 22/11 V 326.]
References
[1] I. VIRANT-KLUN H M T Tomaevi. Sperm Single-Stranded DNA, Detected by Acridine Orange Staining, Reduces Fertilization and Quality of ICSI-Derived Embryos[J]. Journal of Assisted Reproduction and Genetics, 2002, 19 1: 319-328. DOI: 10.1023/a:1016006509036
[2] JUNYAN HAN Kevin B. Fluorescent Indicators for Intracellular pH[J]. Chemical Reviews, 2009, 110 5: 2709-2728. DOI: 10.1021/cr900249z
[3] MARCOS P THOMé. Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy.[J]. Journal of cell science, 2016: 4622-4632. DOI: 10.1242/jcs.195057
Acridine Orange Stain Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hebei Chuanghai Biotechnology Co., Ltd | +86-15350571055 | Sibel@chuanghaibio.com | China | 8737 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | +86-29-81139210 17389186172 | 1077@dideu.com | China | 3999 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21531 | 55 |
| BOC Sciences | +1-631-485-4226 | inquiry@bocsci.com | United States | 19935 | 58 |
| career henan chemical co | +86-0371-86658258 | factory@coreychem.com | China | 29780 | 58 |
| Antai Fine Chemical Technology Co.,Limited | 18503026267 | info@antaichem.com | CHINA | 9631 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 +86-13203830695 | laboratory@coreychem.com | China | 30203 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +86-189 4982 3763 | sales@tnjchem.com | China | 34526 | 58 |
| Guangzhou TongYi biochemistry technology Co.,LTD | tongyibiochem@163.com | China | 2988 | 58 | |
| HANGZHOU LEAP CHEM CO., LTD. | +86-571-87711850 +8619957148339 | market18@leapchem.com | China | 24569 | 58 |
View Lastest Price from Acridine Orange Stain manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-08-07 | ACRIDINE ORANGE
494-38-2
|
$18.00-30.00 | 1KG | 99% | 500KG | Shaanxi Dideu Medichem Co. Ltd | |
 |
2026-06-30 | ACRIDINE ORANGE
494-38-2
|
1KG | 98 | 10000KGS | Shaanxi Dideu New Materials Co. Ltd | ||
 |
2026-03-20 | ACRIDINE ORANGE
494-38-2
|
$10.00 | 100KG | 99% | 100 mt | Hebei Chuanghai Biotechnology Co., Ltd |
-
- ACRIDINE ORANGE
494-38-2
- $18.00-30.00
- 99%
- Shaanxi Dideu Medichem Co. Ltd
-
- ACRIDINE ORANGE
494-38-2
- 98
- Shaanxi Dideu New Materials Co. Ltd
-
- ACRIDINE ORANGE
494-38-2
- $10.00
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
