2-Nitrophenyl-beta-D-galactopyranoside
- CAS No.
- 369-07-3
- Chemical Name:
- 2-Nitrophenyl-beta-D-galactopyranoside
- Synonyms
- ONPG;2-Nitrophenyl;2-NITROPHENYL-B-D-GALACTOPYRANOSIDE;O-NITROPHENYL-B-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactopyranoside;o-Nitrophenyl β-D-Galactopyranoside;o-NPG=ONPG;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D
- CBNumber:
- CB1366392
- Molecular Formula:
- C12H15NO8
- Molecular Weight:
- 301.25
- MDL Number:
- MFCD00063255
- MOL File:
- 369-07-3.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Product description | Number | Pack Size | Price |
| o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate. | 48712-M | 1g | $60.3 |
| o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate. | 48712-M | 5G | $241 |
| 2-Nitrophenyl β-D-galactopyranoside ≥99.0% (HPLC) | 73660 | 1G | $40.3 |
| 2-Nitrophenyl β-D-galactopyranoside ≥99.0% (HPLC) | 73660 | 5G | $142 |
| 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase] >98.0%(HPLC) | N0418 | 1g | $30 |
| More product size | |||
| Melting point | 186-193 °C (dec.) |
|---|---|
| alpha | -70 º (c=1, H2O) |
| Boiling point | 442.39°C (rough estimate) |
| Density | 1.3709 (rough estimate) |
| refractive index | -68.8 ° (C=1, H2O) |
| storage temp. | 2-8°C |
| solubility | DMSO (Slightly), Water (Slightly, Heated, Sonicated) |
| pka | 12.69±0.70(Predicted) |
| form | Powder |
| color | White to very slightly yellow |
| optical activity | [α]20/D 70±1°, c = 1% in H2O |
| Water Solubility | Soluble in water. |
| BRN | 92207 |
| InChI | 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1 |
| InChIKey | KUWPCJHYPSUOFW-RMPHRYRLSA-N |
| SMILES | OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O |
| CAS DataBase Reference | 369-07-3(CAS DataBase Reference) |
| FDA UNII | 8RFX6AT9WP |
| NIST Chemistry Reference | Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3) |
| UNSPSC Code | 12352200 |
| NACRES | NA.77 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H335 | |||||||||
| Precautionary statements | P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362+P364-P403+P233-P501c | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 22-24/25-36-26 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3-10-21 | |||||||||
| HS Code | 29400090 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| NFPA 704 |
|
2-Nitrophenyl-beta-D-galactopyranoside price More Price(71)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 48712-M | o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate. | 369-07-3 | 1g | $60.3 | 2026-04-30 | Buy |
| Sigma-Aldrich | 48712-M | o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem A β-galactosidase substrate for colorimetric and EIA applications; counterpart of widely used pNPP/alkaline phosphatase substrate. | 369-07-3 | 5G | $241 | 2026-04-30 | Buy |
| Sigma-Aldrich | 73660 | 2-Nitrophenyl β-D-galactopyranoside ≥99.0% (HPLC) | 369-07-3 | 1G | $40.3 | 2022-05-15 | Buy |
| Sigma-Aldrich | 73660 | 2-Nitrophenyl β-D-galactopyranoside ≥99.0% (HPLC) | 369-07-3 | 5G | $142 | 2022-05-15 | Buy |
| TCI Chemical | N0418 | 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase] >98.0%(HPLC) | 369-07-3 | 1g | $30 | 2021-12-16 | Buy |
2-Nitrophenyl-beta-D-galactopyranoside Chemical Properties,Uses,Production
Description
2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
Chemical Properties
White Crystalline Solid
Uses
2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.
Definition
ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.
General Description
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
Biochem/physiol Actions
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
Purification Methods
Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]
2-Nitrophenyl-beta-D-galactopyranoside Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| CD Chemical Group Limited | +86-13651442114 | info@codchem.com | China | 17253 | 58 |
| Yunbio Tech Co.,Ltd. | +86-010-60605551 +86-18046518538 | yunbiochem@126.com | China | 321 | 58 |
| Hebei Yanxi Chemical Co., Ltd. | +86-17531190177 | faithe@yan-xi.com | China | 5838 | 58 |
| Hebei Chuanghai Biotechnology Co., Ltd | +86-17732866630 | mina@chuanghaibio.com | China | 18114 | 58 |
| Anhui Ruihan Technology Co., Ltd | +undefined17756083858 | daisy@anhuiruihan.com | China | 954 | 58 |
| Capot Chemical Co.,Ltd. | +86-（0）57185586718 +86-13336195806 | sales@capot.com | China | 29626 | 60 |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-2158073036 | info@dakenam.com | China | 14032 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21531 | 55 |
| Shanghai Zheyan Biotech Co., Ltd. | 18017610038 | zheyansh@163.com | CHINA | 3618 | 58 |
| career henan chemical co | +86-0371-86658258 | sales@coreychem.com | China | 29766 | 58 |
View Lastest Price from 2-Nitrophenyl-beta-D-galactopyranoside manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-08-07 | 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
|
1KG | 98.0% | 500kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | ||
 |
2026-06-30 | 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
|
1kg | 98% | 1000kg | Shaanxi Dideu New Materials Co. Ltd | ||
 |
2025-06-27 | 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
|
1KG | 99% | 500000kg | Hebei Chuanghai Biotechnology Co., Ltd |
-
- 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
- $0.00
- 98.0%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
- 98%
- Shaanxi Dideu New Materials Co. Ltd
-
- 2-Nitrophenyl-beta-D-galactopyranoside
369-07-3
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
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