ChemicalBook >> CAS DataBase List >>4,5-Diaminofluorescein

4,5-Diaminofluorescein

CAS No.
205391-01-1
Chemical Name:
4,5-Diaminofluorescein
Synonyms
DAF-2;5-Diaminofluorescein;5,6-DIAMINOFLUORESCEIN;4,5-Diaminofluorescein;DAF-2;4;5-DIAMINOFLUORESCEIN;4,5-Diaminofluorescein, DAF-2;DAF-2, Fluorescent nitric oxide probe;4,5-Diaminofluorescein-Isopropanol adduct (1:2);2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID;5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
CBNumber:
CB3354894
Molecular Formula:
C20H14N2O5
Molecular Weight:
362.34
MDL Number:
MFCD05865100
MOL File:
205391-01-1.mol
MSDS File:
SDS
Last updated:2026-09-12 18:52:00

4,5-Diaminofluorescein Properties

Boiling point 760.832±60.00 °C(Press: 760.00 Torr)(predicted)
Density 1.696±0.10 g/cm3(Temp: 25 °C; Press: 760 Torr)(predicted)
storage temp. 2-8°C
form powder
pka 9.319±0.20(predicted)
color Brown to black
InChI 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
InChIKey LTYUPYUWXRTNFQ-UHFFFAOYSA-N
SMILES Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O
UNSPSC Code 41116100
NACRES NA.32

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)
GHS07
Signal word  Warning
Hazard statements  H302
Precautionary statements  P264-P270-P301+P312-P330-P501
Hazard Codes  Xn,Xi
Risk Statements  22-36/37/38
Safety Statements  23-26-36
WGK Germany  3
8-10-23
Storage Class 10 - Combustible liquids
NFPA 704
2
2 0

4,5-Diaminofluorescein price More Price(24)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 251500-M DAF-2-CAS205391-01-1-Calbiochem Ahighlysensitiveandspecificfluorescentindicatorforthedirectdetectiono 205391-01-1 1mg $653 2026-04-30 Buy
Sigma-Aldrich 251500-M DAF-2 - CAS 205391-01-1 - Calbiochem A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. 205391-01-1 251500-1MG $591 2024-03-01 Buy
Cayman Chemical 85160 DAF-2 ≥98% 205391-01-1 100μg $68 2026-04-30 Buy
Cayman Chemical 85160 DAF-2 ≥98% 205391-01-1 250μg $154 2026-04-30 Buy
Cayman Chemical 85160 DAF-2 ≥98% 205391-01-1 500μg $291 2026-04-30 Buy
Product number Packaging Price Buy
251500-M 1mg $653 Buy
251500-M 251500-1MG $591 Buy
85160 100μg $68 Buy
85160 250μg $154 Buy
85160 500μg $291 Buy

4,5-Diaminofluorescein Chemical Properties, Uses, Production

Description

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM.

Uses

DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.

Uses

Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.

References

[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI: 10.1248/cpb.46.373
[2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI: 10.1016/s0014-5793(98)00440-2

4,5-Diaminofluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

4,5-Diaminofluorescein Suppliers

Global Suppliers ( 104)
Supplier Tel Email Country ProdList Advantage
Shanghai Aladdin Bio-Chem Technology Co.,LTD 400-6206333 13167063860 anhua.mao@aladdin-e.com China 29985 65
Bide Pharmatech Ltd. 400-164-7117 18317119277 product02@bidepharm.com China 40000 60
Shanghai Macklin Biochemical Co.,Ltd. 15221275939 15221275939 shenlinxing@macklin.cn China 15775 55
Tianjin Being Technology Co., Ltd. 18622939839 tjby921@163.com China 640 55
Shanghai EFE Biological Technology Co., Ltd. 021-65675885 18964387627 info@efebio.com China 9799 58
Shanghai YuanYe Biotechnology Co., Ltd. 15026964105 2881489226@qq.com China 89667 60
Tianjin Kailiqi Biotechnology Co., Ltd. 15076683720 klq@cw-bio.com China 9783 55
Chengdu Huachun Technology Co., Ltd 400-1166-196 15982826727 cdhxsj@163.com China 14571 60
Shanghai Zhen Li Biological Technology Co., Ltd 021-16621358918; 18516310516;16621358918;13045646768 zhenlipharma888@163.com China 19060 58
Bejing Famous Pharmaceutical Technology Co., Ltd. 010-80339217; 13331045123 2559355526@qq.com China 14180 58

View Latest Price from 4,5-Diaminofluorescein manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
DAF-2 pictures 2025-06-07 DAF-2
205391-01-1
5mg >99.00% 5mg Xian confluore Biological Technology Co., Ltd.
  • DAF-2 pictures
  • DAF-2
    205391-01-1
  • $0.00
  • >99.00%
  • Xian confluore Biological Technology Co., Ltd.
2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID 5,6-DIAMINOFLUORESCEIN 4,5-Diaminofluorescein, DAF-2 4,5-Diaminofluorescein-Isopropanol adduct (1:2) 5-Diaminofluorescein DAF-2;4;5-DIAMINOFLUORESCEIN Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5,6-diamino-3',6'-dihydroxy- 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one DAF-2, Fluorescent nitric oxide probe DAF-2 4,5-Diaminofluorescein 205391-01-1 C20H14N2O5 C20H14N2O52C3H8O