DEMECOLCINE
- CAS No.
- 477-30-5
- Chemical Name:
- DEMECOLCINE
- Synonyms
- COLCEMID;COLCHAMINE;omaine;Colcemide;Tropisteron;(S)-1,2,3,10-Tetramethoxy-7-(methylamino)-6,7-dihydrobenzo[a]heptalen-9(5H)-one;omain;Osamin;C-12669;nsc3096
- CBNumber:
- CB3460085
- Molecular Formula:
- C21H25NO5
- Molecular Weight:
- 371.43
- MDL Number:
- MFCD00075459
- MOL File:
- 477-30-5.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 73-75°C |
|---|---|
| alpha | D20 -129.0° (c = 1 in chloroform) |
| Boiling point | 501.11°C (rough estimate) |
| Density | 1.2350 (rough estimate) |
| refractive index | 1.5614 (estimate) |
| storage temp. | 2-8°C |
| solubility | DMSO: 10 mg/mL |
| form | powder |
| pka | 8.48±0.40(Predicted) |
| color | White to off-white |
| Sensitive | Air & Light Sensitive |
| BRN | 2822892 |
| Stability | Hygroscopic |
| InChI | 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3 |
| InChIKey | NNJPGOLRFBJNIW-UHFFFAOYSA-N |
| SMILES | N(C1CCc2c(c(c(c(c2)OC)OC)OC)C3=CC=C(C(=O)C=C31)OC)C |
| FDA UNII | Z01IVE25KI |
| ATC code | L01CC01 |
| UNSPSC Code | 12352207 |
| NACRES | NA.75 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS06 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H300 | |||||||||
| Precautionary statements | P264-P301+P310 | |||||||||
| Hazard Codes | T,T+ | |||||||||
| Risk Statements | 25-26/27/28 | |||||||||
| Safety Statements | 22-24/25-45-36/37/39 | |||||||||
| RIDADR | UN 2811 6.1/PG 2 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | GH0800000 | |||||||||
| F | 8-10-23 | |||||||||
| HazardClass | 6.1(a) | |||||||||
| PackingGroup | II | |||||||||
| Toxicity | LD50 oral in mouse: 25530ug/kg | |||||||||
| NFPA 704 |
|
DEMECOLCINE price More Price(75)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | D1925 | Demecolcine solution 10 μg/mL in HBSS, ACF Qualified, BioXtra | 477-30-5 | 20ml | $68.8 | 2026-04-30 | Buy |
| Sigma-Aldrich | 234109-M | Colcemid - CAS 477-30-5 - Calbiochem Cell synchronization agent. | 477-30-5 | 5mg | $139 | 2026-04-30 | Buy |
| Sigma-Aldrich | 234109-M | Colcemid Cell synchronization agent. | 477-30-5 | 500 mg | $4760 | 2026-04-30 | Buy |
| Sigma-Aldrich | 234109-M | Colcemid Cell synchronization agent. | 477-30-5 | 1 g | $15460 | 2026-04-30 | Buy |
| Sigma-Aldrich | 234109-M | Colcemid Cell synchronization agent. | 477-30-5 | 10 mg | $16040 | 2026-04-30 | Buy |
DEMECOLCINE Chemical Properties,Uses,Production
Description
Colcemid is a colchicine derivative that inhibits tubulin polymerization as potently as colchicine (IC50 = 2.1 and 2.4 μM, respectively) but is less toxic. At very low (nanomolar) concentrations, colcemid suppresses microtubule dynamicity and inhibits cell migration, while at micromolar levels it blocks microtubule assembly, arresting cells in metaphase. Mitotic block by colcemid is used to synchronize cells and for karyotyping in cytogenetic studies. Prolonged exposure to colcemid can activate p53, leading to apoptosis.
Chemical Properties
Faintly Yellow Crystalline Powder
Uses
An antimitotic agent that disrupts microtubles by binding to tubulin and preventing its polymerization. Stimulates the intrinsic GTPase activity of tubulin. Induces apoptosis in several normal and tumor cell lines and activates the JNK/SAPK signaling pathway.
Uses
Inhibitor of spindle fiber formation
Uses
Cell synchronization agent; for chromosome visualization; to induce oocyte enucleation for somatic cell cloning.
Definition
ChEBI: A secondary amino compound that is (S)-colchicine in which the N-acetyl group is replaced by an N-methyl group. Isolable from the autumn crocus, Colchicum autumnale, it is less toxic than olchicine and is used as an antineoplastic.
General Description
Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.
Biochem/physiol Actions
Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.
Safety Profile
Poison by ingestion, intraperitoneal, parenteral, intravenous, and intramuscular routes. Human systemic effects by ingestion: (skin and appendages) hair effects. Human mutation data reported. An experimental teratogen. Experimental reproductive effects. When heated to decomposition it emits toxic fumes of NOx.
Purification Methods
Colcemide is purified by chromatography on silica and eluting with CHCl3/MeOH (9:1), and by recrystallisation from EtOAc/Et2O to form yellow prisms. UV in EtOH has max 243nm ( 30,200) and 350nm ( 16,3000). [Synthesis, IR, NMR, MS: Capraro & Brossi Helv Chim Acta 62 965 1979, Beilstein 8 IV 3319.]
References
[1] ANJUM MUZAFFAR. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids[J]. Journal of Medicinal Chemistry, 1990, 33 2: 567-571. DOI: 10.1021/jm00164a015
[2] C L RIEDER R E P. Colcemid and the mitotic cycle.[J]. Journal of cell science, 1992, 102 ( Pt 3): 387-392. DOI: 10.1242/jcs.102.3.387
[3] MARY ANN JORDAN Leslie W. Microtubules as a target for anticancer drugs[J]. Nature Reviews Cancer, 2004, 4 4: 253-265. DOI: 10.1038/nrc1317
[4] JOZEF CURLEJ Peter C Jozef Bulla. Occurrence of chromosomal aneuploidy in rabbit oocytes and embryos at different developmental stages.[J]. Zygote, 2010, 18 3: 203-207. DOI: 10.1017/s0967199409990207
[5] HAILING YANG F C Anutosh Ganguly. Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs.[J]. The Journal of Biological Chemistry, 2010: 32242-32250. DOI: 10.1074/jbc.m110.160820
[6] JANE BAYANI Jeremy A S. Preparation of Cytogenetic Specimens from Tissue Samples[J]. Current Protocols in Cell Biology, 2004, 23 1. DOI: 10.1002/0471143030.cb2202s23
[7] ANNA A SABLINA. p53 activation in response to microtubule disruption is mediated by integrin-Erk signaling[J]. Oncogene, 2001, 20 8: 899-909. DOI: 10.1038/sj.onc.1204156
DEMECOLCINE Preparation Products And Raw materials
DEMECOLCINE Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| LGM Pharma | 1-(800)-881-8210 | inquiries@lgmpharma.com | United States | 2110 | 70 |
| Chemsky（shanghai）International Co.,Ltd. | 021-50135380 | shchemsky@sina.com | China | 32273 | 50 |
| Sichuan Kulinan Technology Co., Ltd | 400-1166-196 18981987031 | cdhxsj@163.com | China | 11674 | 57 |
| Dalian Meilun Biotech Co., Ltd. | 0411-62910999 13889544652 | sales@meilune.com | China | 4765 | 58 |
| Hangzhou Sage Chemical Co., Ltd. | +86057186818502 13588463833 | info@sagechem.com | China | 10265 | 58 |
| Beijing HuaMeiHuLiBiological Chemical | 010-56205725 | waley188@sohu.com | China | 12323 | 58 |
| Guangzhou Isun Pharmaceutical Co., Ltd | 020-39119399 18927568969 | isunpharm@qq.com | China | 4773 | 55 |
| Shanghai Macklin Biochemical Co.,Ltd. | 15221275939 15221275939 | shenlinxing@macklin.cn | China | 15775 | 55 |
| Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51389 | 80 |
View Lastest Price from DEMECOLCINE manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-02 | DEMECOLCINE
477-30-5
|
$1.00-8.00 | 1KG | 99% | Henan Fengda Chemical Co., Ltd | ||
 |
2026-08-16 | Colcemid
477-30-5
|
$31.00-156.00 | 99.65% | 10g | TargetMol Chemicals Inc. | ||
 |
2026-08-07 | DEMECOLCINE
477-30-5
|
$1.00-45.00 | 1KG | 99% | 420000KG | Shaanxi Dideu Medichem Co. Ltd |
-
- DEMECOLCINE
477-30-5
- $1.00-8.00
- 99%
- Henan Fengda Chemical Co., Ltd
-
- Colcemid
477-30-5
- $31.00-156.00
- 99.65%
- TargetMol Chemicals Inc.
-
- DEMECOLCINE
477-30-5
- $1.00-45.00
- 99%
- Shaanxi Dideu Medichem Co. Ltd
