Sulforhodamine B
- CAS No.
- 3520-42-1
- Chemical Name:
- Sulforhodamine B
- Synonyms
- ACID RED 52;ACID RED;CI 45100;LISSAMINE RHODAMINE B;sulforhodamine b sodium salt;red106;AKA106;XYLENE RED;KITON RED S;LISSAMINE RHODAMINE
- CBNumber:
- CB6223683
- Molecular Formula:
- C27H31N2NaO7S2
- Molecular Weight:
- 582.66
- MDL Number:
- MFCD00010180
- MOL File:
- 3520-42-1.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Density | 1.472[at 20℃] |
|---|---|
| storage temp. | room temp |
| solubility | methanol: 1 mg/mL |
| Colour Index | 45100 |
| form | powder |
| color | Brown to Very Dark Purple |
| biological source | synthetic (organic) |
| Water Solubility | 95.3g/L at 20℃ |
| λmax | 554 nm |
| ε(extinction coefficient) | ≥85000 at 554-560nm in 0.1 M NaOH in methanol at 0.005g/L |
| BRN | 3886008 |
| Major Application |
diagnostic assay manufacturing hematology histology |
| Cosmetics Ingredients Functions |
COLORANT HAIR DYEING |
| InChIKey | XCRMYPBRGZRYEU-UHFFFAOYSA-N |
| SMILES | S(C1C=C(S(O)(=O)=O)C=CC=1C1C2=CC=C(N(CC)CC)C=C2[O+]=C2C=C(N(CC)CC)C=CC=12)([O-])(=O)=O.[NaH] |
| LogP | -2.2 at 20℃ |
| EWG's Food Scores | 1-3 |
| FDA UNII | 821LWZ3R6R |
| EPA Substance Registry System | C.I. Acid Red 52 (3520-42-1) |
| UNSPSC Code | 12352207 |
| NACRES | NA.75 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H335-H319 | |||||||||
| Precautionary statements | P264-P280-P305+P351+P338-P337+P313P-P264-P280-P302+P352-P321-P332+P313-P362 | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 22-24/25-26-36/37/39 | |||||||||
| WGK Germany | 2 | |||||||||
| RTECS | BP6750000 | |||||||||
| F | 8 | |||||||||
| TSCA | TSCA listed | |||||||||
| HS Code | 32129000 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| REACH Registrations | Active | |||||||||
| NFPA 704 |
|
Sulforhodamine B price More Price(57)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 230162 | Sulforhodamine B Dye content 75 % | 3520-42-1 | 1g | $41.9 | 2026-04-30 | Buy |
| Sigma-Aldrich | 230162 | Sulforhodamine B Dye content 75 % | 3520-42-1 | 5g | $129 | 2026-04-30 | Buy |
| TCI Chemical | F0143 | Food Red No. 106 | 3520-42-1 | 25g | $108 | 2026-04-30 | Buy |
| TCI Chemical | A0600 | Acid Red 52 | 3520-42-1 | 25g | $81 | 2026-04-30 | Buy |
| Cayman Chemical | 31539 | Sulforhodamine B (sodium salt) (technical grade) ≥75% | 3520-42-1 | 5mg | $65 | 2026-04-30 | Buy |
Sulforhodamine B Chemical Properties, Uses, Production
Description
Sulforhodamine B is a membrane-impermeant polar tracer.
Chemical Properties
This substance is a deep red powder. It is easily soluble in water, forming a bluish-red solution with yellow fluorescence. When sodium hydroxide solution is added, it also appears bluish-red. Additionally, it is soluble in ethanol. In concentrated sulfuric acid, it turns orange-yellow and red after dilution.
Uses
Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing
Uses
Sulforhodamine B sodium salt has been used:
- for relative total cell mass quantification
- to investigate the viscosity of coacervates
- as a molecular rotor for microviscosity determination in aerosol droplets
Application
Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material.
Definition
ChEBI: Sulforhodamine B is an organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion.
Preparation
4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt.
General Description
Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.
Flammability and Explosibility
Not classified
Biochem/physiol Actions
Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.
Properties and Applications
bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.
| Standard | Light Fastness | Soaping | Persperation Fastness | Oxygen bleaching | Fastness to seawater | |||
| Fading | Stain | Fading | Stain | Fading | Stain | |||
| ISO | 3 | 3-4 | 3 | 4 | 1 | 2 | 3 | 2 |
| AATCC | 2-3 | 3 | 3 | 3 | 1 | 2 | 4 | 2-3 |
Sulforhodamine B Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hubei Yiling Biotechnology Co., Ltd | 027-65526929 17771822910 | hxry001@foxmail.com | China | 50 | 58 |
| Hubei Zhonglong Kangsheng fine chemical Co.,Ltd | 027-83850125 15927542930 | 3321236220@qq.com | China | 996 | 58 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| Alfa Aesar | 400-6106006 | saleschina@alfa-asia.com | China | 30103 | 84 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Beijing HwrkChemical Technology Co., Ltd | 89508211 18501085097 | sales.bj@hwrkchemical.com | China | 9407 | 55 |
| Energy Chemical | 400-0056266 | sales8178@energy-chemical.com | China | 44850 | 61 |
| Beijing Ouhe Technology Co., Ltd | 13552068683 13522913783 | 2355560935@qq.com | China | 11777 | 60 |
| Sichuan Kulinan Technology Co., Ltd | 400-1166-196 18981987031 | cdhxsj@163.com | China | 11674 | 57 |
View Latest Price from Sulforhodamine B manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-18 | SULFORHODAMINE B
3520-42-1
|
0.99 | RongNa Biotechnology Co.,Ltd | ||||
 |
2026-09-02 | Sulforhodamine B
3520-42-1
|
$1.00-6.00 | 1KG | 99% | Henan Fengda Chemical Co., Ltd | ||
 |
2026-07-15 | Sulforhodamine B sodium salt
3520-42-1
|
$29.00 | 99.67% | 10g | TargetMol Chemicals Inc. |
-
- SULFORHODAMINE B
3520-42-1
- 0.99
- RongNa Biotechnology Co.,Ltd
-
- Sulforhodamine B
3520-42-1
- $1.00-6.00
- 99%
- Henan Fengda Chemical Co., Ltd
-
- Sulforhodamine B sodium salt
3520-42-1
- $29.00
- 99.67%
- TargetMol Chemicals Inc.
