| Company Name: |
Alfa Aesar |
| Tel: |
400-6106006 |
| Email: |
saleschina@alfa-asia.com |
- Sulforhodamine B
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- $1.00
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2026-10-10
- CAS:3520-42-1
- Min. Order: 1Kg
- Purity: 98%
- Supply Ability: 20T
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| | Sulforhodamine B Basic information |
| | Sulforhodamine B Chemical Properties |
| density | 1.472[at 20℃] | | storage temp. | room temp | | solubility | methanol: 1 mg/mL | | Colour Index | 45100 | | form | powder | | color | Brown to Very Dark Purple | | biological source | synthetic (organic) | | Water Solubility | 95.3g/L at 20℃ | | λmax | 554 nm | | ε(extinction coefficient) | ≥85000 at 554-560nm in 0.1 M NaOH in methanol at 0.005g/L | | ex/em | λex 566, 355 nm; λem 584 nm in water | | BRN | 3886008 | | Major Application | diagnostic assay manufacturing
hematology
histology | | Cosmetics Ingredients Functions | COLORANT
HAIR DYEING | | InChIKey | XCRMYPBRGZRYEU-UHFFFAOYSA-N | | SMILES | S(C1C=C(S(O)(=O)=O)C=CC=1C1C2=CC=C(N(CC)CC)C=C2[O+]=C2C=C(N(CC)CC)C=CC=12)([O-])(=O)=O.[NaH] | | LogP | -2.2 at 20℃ | | EPA Substance Registry System | C.I. Acid Red 52 (3520-42-1) |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 22-24/25-26-36/37/39 | | WGK Germany | 2 | | RTECS | BP6750000 | | F | 8 | | TSCA | TSCA listed | | HS Code | 32129000 | | Storage Class | 11 - Combustible Solids |
| | Sulforhodamine B Usage And Synthesis |
| Description | Sulforhodamine B is a membrane-impermeant polar tracer. | | Chemical Properties | This substance is a deep red powder. It is easily soluble in water, forming a bluish-red solution with yellow fluorescence. When sodium hydroxide solution is added, it also appears bluish-red. Additionally, it is soluble in ethanol. In concentrated sulfuric acid, it turns orange-yellow and red after dilution. | | Uses | Sulforhodamine B Sodium Salt is a rhodamine dye widely used in nonpermanent tattoos. Studies show that Sulforhodamine B can be used as a potential new therapeutic agent for the treatment of the acute respiratory distress syndrome. Dyes and metabolites, Environmental Testing | | Uses | Sulforhodamine B sodium salt has been used:
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for relative total cell mass quantification
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to investigate the viscosity of coacervates
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as a molecular rotor for microviscosity determination in aerosol droplets
| | Application | Sulforhodamine B (SRB) is known for use in SRB assay for the determination of in vitro cytotoxicity through staining and the observation of live cells, proliferation and drug screening.
It has been used in the SRB assay to evaluate the effect of mouthwashes on protein content in primary cultures of human fibroblasts.
It is also suitable as a microscopic tracer for the assessment of blood-brain barrier integrity by post mortem inspection of frozen sections. SRB is useful as a caries detector dye with subsequent inspection of histological material. | | Definition | ChEBI: Sulforhodamine B is an organic sodium salt having 4-[3,6-bis(diethylamino)-2,7-dimethylxanthenium-9-yl]benzene-1,3-disulfonate as the counterion. | | Preparation | 4-Formylbenzene-1,3-disulfonic acid and 3-(Diethylamino)phenol condensation, the products with sulfuric acid dehydration, and ferric chloride oxidation, and finally into sodium salt. | | General Description | Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination. | | Flammability and Explosibility | Not classified | | Biochem/physiol Actions | Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening. | | Properties and Applications | bright blue light pink. Dark red light brown powder. Soluble in water, in blue light red, with fluorescence, also soluble in alcohol. The strong sulfuric acid dyes is orange, diluted for red, with sodium hydroxide solution is blue light red. Mainly used for wool, silk and polyamide fiber dyeing, also can be used for wool and a variety of fabric dyeing, still can be in wool and silk fabric printing directly, also can used for leather dyeing.
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Standard
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Light Fastness
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Soaping
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Persperation Fastness
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Oxygen bleaching
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Fastness to seawater
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Fading
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Stain
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Fading
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Stain
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Fading
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Stain
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ISO
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3
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3-4
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3
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4
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1
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2
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3
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2
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AATCC
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2-3
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3
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3
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3
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1
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2
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4
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2-3
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| | Sulforhodamine B Preparation Products And Raw materials |
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