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PyBOP

PyBOP Structure
PyBOP structure
CAS No.
128625-52-5
Chemical Name:
PyBOP
Synonyms
PYBOP;N-[(dimethylamino)-3-oxo-1H-1,2,3-triazolo[4,5-b]pyridin-1-yl-methylene]-N-methylmethanaminium hexafluorophosphate;BOP REAGENT;Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate;(BENZOTRIAZOL-1-YLOXY)TRIPYRROLIDINOPHOSPHONIUM HEXAFLUOROPHOSPHATE;BYPOP;BENZOTRIAZOLE-1-YL-OXY-TRIS-PYRROLIDINO-PHOSPHONIUM HEXAFLUOROPHOSPHATE;((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tri(pyrrolidin-1-yl)phosphoniuM hexafluorophosphate(V);BENZOTRIAZOL-1-YLOXYTRIS(PYRROLIDINO)PHOSPHONIUM HEXAFLUOROPHOSPHATE;1H-BENZOTRIAZOL-1-YLOXYTRIPYRROLIDINOPHOSPHONIUM HEXAFLUOROPHOSPHATE
CBNumber:
CB6434717
Molecular Formula:
C18H28F6N6OP2
Molecular Weight:
520.39
MDL Number:
MFCD00077411
MOL File:
128625-52-5.mol
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-09-08 19:26:36
Request For Quotation

PyBOP Properties

Melting point 154-156 °C (dec.)(lit.)
Density 1.438 at 20℃
vapor pressure 0-0Pa at 20-50℃
storage temp. 2-8°C
solubility methanol: 25 mg/mL, clear
form Fine Crystalline Powder
color White to off-white
Sensitive Moisture Sensitive
BRN 3584612
Major Application peptide synthesis
InChI InChI=1S/C18H28N6OP.F6P/c1-2-10-18-17(9-1)19-20-24(18)25-26(21-11-3-4-12-21,22-13-5-6-14-22)23-15-7-8-16-23;1-7(2,3,4,5)6/h1-2,9-10H,3-8,11-16H2;/q+1;-1
InChIKey BYCLGHOAELJLOF-UHFFFAOYSA-M
SMILES [P+](ON1N=NC2C=CC=CC1=2)(N1CCCC1)(N1CCCC1)N1CCCC1.[P-](F)(F)(F)(F)(F)F
LogP -0.3 at 26℃ and pH3.5-3.7
CAS DataBase Reference 128625-52-5(CAS DataBase Reference)
UNSPSC Code 12352107
NACRES NA.22

SAFETY

Risk and Safety Statements

Symbol(GHS)  Exclamation Mark (GHS07)Environment (GHS09)
GHS07,GHS09
Signal word  Warning
Hazard statements  H302-H317-H410
Precautionary statements  P261-P264-P273-P280-P301+P312-P302+P352
PPE dust mask type N95 (US), Eyeshields, Gloves
Hazard Codes  Xi
Risk Statements  37/38-44-36/37/38
Safety Statements  26-36-24/25-37/39
RIDADR  UN 1325 4.1/PG 2
WGK Germany  3
F  8-10-21
Hazard Note  Irritant
HS Code  29339980
Storage Class 11 - Combustible Solids
Hazard Classifications Acute Tox. 4 Oral
Aquatic Acute 1
Aquatic Chronic 1
Skin Sens. 1
REACH Registrations Active
NFPA 704
1
2 0

PyBOP price More Price(106)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich 8.51009 PyBOP Novabiochem? 128625-52-5 25g $135 2026-04-30 Buy
Sigma-Aldrich 8.51009 PyBOP Novabiochem? 128625-52-5 100g $404 2026-04-30 Buy
Sigma-Aldrich 8.51009 PyBOP® Novabiochem® 128625-52-5 500 g $1450 2026-04-30 Buy
Sigma-Aldrich 8.51009 PyBOP® Novabiochem® 128625-52-5 1 kg $2440 2026-04-30 Buy
Sigma-Aldrich 8.51009 PyBOP® Novabiochem® 128625-52-5 5 kg $9810 2026-04-30 Buy
Product number Packaging Price Buy
8.51009 25g $135 Buy
8.51009 100g $404 Buy
8.51009 500 g $1450 Buy
8.51009 1 kg $2440 Buy
8.51009 5 kg $9810 Buy

PyBOP Chemical Properties,Uses,Production

Chemical Properties

Off-White Solid

Uses

One of the most convenient coupling reagents for peptide synthesis without carcinogenic by-products. It replaces BOP reagent. This is especially suitable for solid-phase peptide synthesis, avoids racemization.

Uses

1H-Benzotriazol-1-yloxytri(1-pyrrolidinyl) phosphonium hexafluorophosphate (PyBOP) is a peptide coupling reagent utilized in solid phase peptide synthesis. It is also used as a substitute for the (benzotriazol-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate (BOP) reagent.

Uses

PyBOP is a reagent for: Synthesis of single labeled oligonucleotides that fluoresce upon matched hybridization
Generation of endocrine disruptor chemical binders
Cycloaddition to template-assembled multivalent peptide conjugates

Definition

PyBOP (benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate) coupling reagent was selected for the peptidic bond formation. This selection was because PyBOP is efficient and could generate less toxic byproducts. PyBOP is an efficient coupling reagent utilized in solid-phase peptide synthesis. It reacts similarly to BOP, but its byproduct is not highly carcinogenic. PyBOP was utilized in the solid-phase synthesis of the tetrapeptide H-Ile-Ala-Leu-Pro-OH on 2-chlorotrityl resin. In contrast to uronium-based coupling reagents, PyBOP does not form guanidinated byproducts when used in excess. This makes it useful in fragment condensation reactions and head-to-tail cyclizations[1].

General Description

(Benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate (PyBOP) is an activator of carboxyl group commonly used in peptide synthesis. It also participates in the carboxylic acid esterification. It shows characteristics similar to benzotriazol-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate (BOP) and can be utilized as an alternative to BOP in peptide bond formation.

reaction suitability

reaction type: Coupling Reactions

References

[1] Maharani, R. et al. “Good coupling performance of PyBOP in the solid-phase synthesis of tetrapeptide, OH-Pro-Leu-Ala-Ileu-NH2.” 2017. 0.

PyBOP Preparation Products And Raw materials

Raw materials

Preparation Products

PyBOP Suppliers

Global( 609)Suppliers
Supplier Tel Email Country ProdList Advantage
Suzhou Highfine Biotech Co., Ltd 0512-69209928 18796809688 zhouyingxiang@highfine.com China 472 75
Shanghai Jifeng Biotechnology Co., Ltd. 021-61263377 15023907684 15800684150@163.com China 7994 58
Gill polypeptide biopharmaceutical (dalian) co., LTD 021-61263455 13167021076 amy@glschina.com China 9699 58
Jiangsu Jitai Peptide Technology Co., Ltd. 051580855066 13770132022 sales05@gtaipeptide.com China 2675 58
Binhai gill polypeptide co. LTD 021-61263389 13524856427 Joetang@glschina.com China 9927 58
Lianyungang Guanxin Pharmaceutical Technology Co., Ltd 15351860687 1462699602@qq.com China 36 58
Aikon International Limited 025-58851090 13913916777 sales01@aikonchem.com China 15947 58
Shaoxing Jiufu New Material Technology Co., LTD 0575-87095566 18067860628 sales@sxjiufu.com China 413 58
Nantong Subei Chemical Raw Materials Co., Ltd. 0513-85290972 18762756250 ntsbhg@163.com China 9277 58
Xinghua Advanced Materials (Suzhou) Co., Ltd. 13816002113 68249111@qq.com China 34 58
Supplier Advantage
Suzhou Highfine Biotech Co., Ltd 75
Shanghai Jifeng Biotechnology Co., Ltd. 58
Gill polypeptide biopharmaceutical (dalian) co., LTD 58
Jiangsu Jitai Peptide Technology Co., Ltd. 58
Binhai gill polypeptide co. LTD 58
Lianyungang Guanxin Pharmaceutical Technology Co., Ltd 58
Aikon International Limited 58
Shaoxing Jiufu New Material Technology Co., LTD 58
Nantong Subei Chemical Raw Materials Co., Ltd. 58
Xinghua Advanced Materials (Suzhou) Co., Ltd. 58

Related Questions

Ask a question
Q:Is PyBOP harmful?Anonymous - Aug 19,2025
A:PyBOP is a substitute for the BOP reagent that avoids the formation of the carcinogenic waste product HMPA. Thermal hazard analysis by differential scanning calorimetry shows PyBOP is potentially explosive.
Q:How is PyBOP synthesized?Anonymous - Aug 19,2025
A: PyBOP (benzotriazol-1-yloxytripyrrolidinophosphonium hexafluorophosphate) can be prepared from 1-hydroxybenzotriazole and a chlorophosphonium reagent under basic conditions. It is a reagent used in peptide synthesis.

View Lastest Price from PyBOP manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate pictures 2026-09-11 Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate
128625-52-5
 1KG 98%min 30tons/month WUHAN FORTUNA CHEMICAL CO., LTD
Benzotriazol-1-yl-oxytripyrrolidino-phosphonium Hexafluorophosphate pictures 2026-09-08 Benzotriazol-1-yl-oxytripyrrolidino-phosphonium Hexafluorophosphate
128625-52-5
 100KG 99% 20 T Hangzhou Verychem Science And Technology Co.Ltd
PyBOP pictures 2026-08-26 PyBOP
128625-52-5
 $240.00 1kg 99% 99999 Rongchuang International
  • PyBOP pictures
  • PyBOP
    128625-52-5
  • $240.00
  • 99%
  • Rongchuang International

PyBOP Spectrum

Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate(128625-52-5)1HNMRBenzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate(128625-52-5)IRBenzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate(128625-52-5)Raman

128625-52-5(PyBOP)Related Search:

DEPBT Bis(2-oxo-3-oxazolidinyl)phosphinic chloride TSTU 5-Chloro-1-[bis(dimethylamino)methylene]-1H-benzotriazolium 3-oxide hexafluorophosphate Bromo-tris-pyrrolidino-phosphonium hexafluorophosphate
Diphenylphosphoryl azide 1H-Benzotriazole Hexafluorophosphoric acid Potassium hexafluorophosphate Lithium hexafluorophosphate
Sodium hexafluorophosphate Hexafluorophosphate TRIS(DIETHYLAMINO)PHOSPHINE 1-Hydroxybenzotriazole hydrate 1H-Benzotriazol-1-yloxytris(dimethylamino)phosphonium Hexafluorophosphate
Hexamethylphosphorous triamide Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate

1of4

pybop?/td> BENZOTRIAZOL-1-YL-OXYTRIPYRROLIDINPHOSPHONIUM HEXAFLUOROPHOSPHATE BENZOTRIAZOLE-1-YL-OXY-TRIS-DIMETHYLAMINOPHOPHONIUM BENZOTRIAZOLE-1-YL-OXY-TRIS-(DIMETHYLAMINO)-PHOPHONIUM HEXAFLUOROPHOSPHATE BENZOTRIAZOLE-1-YL-OXY-TRIS-(DIMETHYLAMINO)-PHOSPHONIUM BENZOTRIAZOLE-1-YL-OXY-TRIS-(DIMETHYLAMINO)-PHOSPHONIUM HEXAFLUOROPHOSPHATE BENZOTRIAZOLE-1-YL-OXY-TRIS-PYRRORIDINO-PHOSPHONIUM HEXAFLUOROPHOSPHATE HEXAFLUOROPHOSPHATE BOP CASTROS REAGENT CASTRO'S REAGENT 1H-BENZOTRIAZOL-1-YLOXYTRIPYRROLIDINOPHOSPHONIUM HEXAFLUOROPHOSPHATE PYBOP PYBOP(R) Benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluoroph Benzotriazol-1-yloxytri(1-pyrrolidinyl)phosphonium hexafluorophosphate, 98% PYBOP(R),99% (Benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate,PyBOP Benzotriazol-1-yl-ox (Benzotriazol-1-yloxy)tripyrrolidinophosphonium he 1H-Benzotriazol-1-yloxytri(1-pyrrolidinyl)phosphonium hexafluorophosphate, 98% PyBOP (OR) Benzotriazol-1-yloxy tripyrrolidinophosphoniuM hexafluorophosphate PYBOP, 99% 5GR ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tri(pyrrolidin-1-yl)phosphoniuM hexafluorophosphate(V) [(1H-Benzotriazol-1-yl)oxy][tri(pyrrolidin-1-yl)]phosphonium hexafluorophosphate (1H-Benzotriazol-1-yloxy)(diMethylaMino)di(pyrrolidin-1-yl)phosphoniuM Phosphorus(1+),[1-(hydroxy-kO)-1H-benzotriazolato]tri-1-pyrrolidinyl-,(T-4)-, hexafluorophosphate(1-) (1:1) (1H-1,2,3-benzotriazol-1-yloxy)tris(pyrrolidin-1-yl)phosphanium BENZOTRIAZOL-1-YL-OXYTRIPYRROLIDINO-PHOS PHONIUM (BENZOTRIAZOL-1-YLOXY)TRIPYRROLIDINOPHOSPHONIUM F6 PHOSPHATE PYBOP [BENZOTRIAZOL-1-YLOXYTRIPYRROLIDINOPHOSPHONIUM HEXAFLUOROPHOSPHATE] Benzotriazol-1-yloxytris(pyrrolidino)phosphoniumh Benzotriazol-1-yloxytris-(pyrrolidino)-phosphonium Benzotriazol-1-yloxytripyrroli Benzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphateBenzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate Benzotriazol-1-yloxytris(pyrrolidino)phosphonium Hexafluorophosphate (PyBOP) Benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphat PYBOP ((1H-BENZOTRIAZOL-1-YLOXY)TRIPYRROLIDINOPHOSPHONIUMHEXAFLUOROPHOSPHATE) Benzotriazole-1-ly-oxy-tris-pyrrolidinophosphonium hexafluorphosphate 1H-Benzotriazol-1-yloxytripyrrolidinophosphonium Hexafluorophosphate [Coupling Reagent for Peptide] Benzotriazol-1-yl-oxytrispyrrolidinophosphonium hexafluorophosphate, 97+% Benzotriazole-1-yl-oxy-trispyrrolidinophosphonium hexafluorophosphate ,99% (Benzotriazol-1-yloxy)tripyrrolidinophosphoniuM hexafluorophosphate 98% 1H-Benzotriazol-1-yloxytripyrrolidinophosphoniuM hexafluorophosphate, 98.0%(LC) 1 h benzotriazole - 1 - three pyrrole alkyl six fluoride phosphate PyBOP base oxygen enzotriazole-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (Benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate≥ 99% (HPLC) PyBOP Novabiochem 1-Benzotriazolyloxytripyrrolidinylphosphonium hexafluorophosphate Unable to generate chemical name BENZOTRIAZOLYL-N-OXYTRIS-(DIMETHY Benzotriazol-1-yloxytrispyrrolidinophosphonium hexafluorophosphate, 99%, for synthesis 1H-Benzotriazol-1-yloxytripyrrolidinophosphonium Hexafluorophosphate [Coupling Reagent for Peptide]> PYBOP 25 G PYBOP 100 G idinophosphonium hexafL oxytripyrroL Phosphorus(1+), [1-(hydroxy-κO)-1H-benzotriazolato]tri-1-pyrrolidinyl-, (T-4)-, hexafluorophosphate(1-) (1:1) PyBOP, 99% min.