3,3'-Diaminobenzidine tetrahydrochloride hydrate
- CAS No.
- 868272-85-9
- Chemical Name:
- 3,3'-Diaminobenzidine tetrahydrochloride hydrate
- Synonyms
- DAB，hydrate;DAB·4HCl Hydrate;Diaminobenzidine-4HCl-xH2O;DAB TETRAHYDROCHLORIDE HYDRATE;FLUORESCENT PROTEIN QUANTITATION KIT;3,4-DiaMonobenzidine tetrahydrchloride;4'-Biphenyltetraminetetrahydrochloride;RNA LOADING BUFFER WITH ETHIDIUM BROMIDE;3,3'-diaminobiphenylaminetrahydrochloride;3,3-Diaminobenzidine Tetrahydrochloride Hydrate
- CBNumber:
- CB6874674
- Molecular Formula:
- C12H17ClN4O
- Molecular Weight:
- 268.75
- MDL Number:
- MFCD08273058
- MOL File:
- 868272-85-9.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 300°C |
|---|---|
| storage temp. | room temp |
| solubility | H2O: soluble50mg/mL |
| form | Powder |
| color | White to Amber to Dark purple |
| Water Solubility | Soluble at 5%(w/v) in water |
| Major Application |
diagnostic assay manufacturing hematology histology |
| InChI | InChI=1S/C12H14N4.ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;/h1-6H,13-16H2;1H;1H2 |
| InChIKey | YOEDEMAWKGDBAW-UHFFFAOYSA-N |
| SMILES | NC1=C(C=CC(C2C=CC(N)=C(N)C=2)=C1)N.Cl.O |
| FDA UNII | Z3KH58591V |
| UNSPSC Code | 41116105 |
| NACRES | NA.47 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319 | |||||||||
| Precautionary statements | P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313 | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Hazard Codes | Xn,T | |||||||||
| Risk Statements | 45-68-36-22 | |||||||||
| Safety Statements | 22-24/25-45-53-26 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | DV8753000 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 29215900 | |||||||||
| Storage Class | 6.1C - Combustible acute toxic Cat.3 toxic compounds or compounds which causing chronic effects |
|||||||||
| Hazard Classifications | Acute Tox. 4 Oral Carc. 1B Eye Irrit. 2 Muta. 2 |
|||||||||
| NFPA 704 |
|
3,3'-Diaminobenzidine tetrahydrochloride hydrate price More Price(49)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 32750 | 3,3′-Diaminobenzidine tetrahydrochloride hydrate for spectrophotometric det. of Se, ≥97.5% | 868272-85-9 | 5G | $111 | 2026-04-30 | Buy |
| Sigma-Aldrich | 32750 | 3,3′-Diaminobenzidine tetrahydrochloride hydrate for spectrophotometric det. of Se, ≥97.5% | 868272-85-9 | 25G | $471 | 2026-04-30 | Buy |
| Sigma-Aldrich | 32750 | 3,3′-Diaminobenzidine tetrahydrochloride hydrate for spectrophotometric det. of Se, ≥97.5% | 868272-85-9 | 1G | $34.4 | 2022-05-15 | Buy |
| TCI Chemical | D0078 | 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate >98.0%(HPLC)(N) | 868272-85-9 | 5g | $98 | 2026-04-30 | Buy |
| TCI Chemical | D0078 | 3,3'-Diaminobenzidine Tetrahydrochloride Hydrate >98.0%(HPLC)(N) | 868272-85-9 | 25g | $391 | 2026-04-30 | Buy |
3,3'-Diaminobenzidine tetrahydrochloride hydrate Chemical Properties, Uses, Production
Chemical Properties
Grey to brownish-purple crystalline powder
Uses
3,3'-diaminobenzidine-tetrahydrochloride-dihydrate(DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
Uses
3,3′-Diaminobenzidine tetrahydrochloride hydrate can be used in HRP-based assays suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).
General Description
3,3′-Diaminobenzidine tetrahydrochloride hydrate, also, known as DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.
Biochem/physiol Actions
In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.
3,3'-Diaminobenzidine tetrahydrochloride hydrate Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| WuYan Pharmaceutical Technology (Shanghai) Co., Ltd. | 18621565901 | sales@wuyanpharm.com | China | 139 | 55 |
| Nanjing King-pharm Co.,LTD. | 025-58748998 18951874644 | elsa.cheng@chemsigma.com | China | 137 | 65 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Beijing HwrkChemical Technology Co., Ltd | 89508211 18501085097 | sales.bj@hwrkchemical.com | China | 9407 | 55 |
| Energy Chemical | 400-0056266 | sales8178@energy-chemical.com | China | 44850 | 61 |
| Adamas Reagent, Ltd. | 400-6009262 15618770481 | yhx@titansci.com | China | 14098 | 59 |
| Sichuan Kulinan Technology Co., Ltd | 400-1166-196 18981987031 | cdhxsj@163.com | China | 11674 | 57 |
| Tianjin heowns Biochemical Technology Co., Ltd. | 400 638 7771 | sales@heowns.com | China | 14412 | 57 |
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