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4-Nitrophenyl-N-acetyl-beta-D-glucosaminide

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide structure

CAS No.: 3459-18-5

Chemical Name:: 4-Nitrophenyl-N-acetyl-beta-D-glucosaminide

Synonyms: p-Nitrophenyl 2-Acetamido-2-deoxy-β-D-glucopyranoside;GLCNAC1-B-PNP;PNP-BETA-D-GLCNAC;4-Nitorphenyl--N-acetyl-D-;PNP N-ACETYL-BETA-D-GLUCOSAMINIDE;4-Nitrophenyl 2-acetamido-2-deoxy-;p-Nitrophenyl-N-acetylglucosaminide;4-Nitrophenyl 2-acetamido-2-deoxy-β;P-Nitrophenyl-2-Acetamido-2-Deoxy-B-D-;2-NITROPHENYL-N-ACETYL-β-D-GLUCOSAMINE

CBNumber:: CB7445022

Molecular Formula:: C14H18N2O8

Molecular Weight:: 342.3

MDL Number:: MFCD00063696

MOL File:: 3459-18-5.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-09-12 18:54:57

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4-Nitrophenyl-N-acetyl-beta-D-glucosaminide Properties

Melting point	210-212 °C
Boiling point	477.77°C (rough estimate)
alpha	-14~-18°(D/20℃) (c=0,H2O)
Density	1.3038 (rough estimate)
refractive index	1.5110 (estimate)
storage temp.	-20°C
solubility	H₂O: 5 mg/mL, clear, colorless to very faintly yellow
form	Fine Powder
pka	12.76±0.70(Predicted)
color	White to off-white
Water Solubility	water: 5mg/mL, clear, colorless to very faintly yellow
λmax	400nm(Phosphate buffer sol.)(lit.)
BRN	96193
InChI	InChI=1/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/s3
InChIKey	OMRLTNCLYHKQCK-DOSMMQPLNA-N
SMILES	O(C1C=CC(N(=O)=O)=CC=1)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(=O)C \|&1:10,12,15,17,19,r\|
CAS DataBase Reference	3459-18-5(CAS DataBase Reference)
FDA UNII	GVY9AA7Y5C
UNSPSC Code	12352204
NACRES	NA.77

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H315-H319-H335
Precautionary statements	P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P362+P364-P403+P233-P501c
PPE	Eyeshields, Gloves, type N95 (US)
Hazard Codes	Xi
Safety Statements	22-24/25
WGK Germany	3
F	10-21
HS Code	29329990
Storage Class	11 - Combustible Solids

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide price More Price(56)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	N9376	4-Nitrophenyl N-acetyl-β-D-glucosaminide ≥99% (TLC)	3459-18-5	1g	$300	2026-04-30	Buy
Sigma-Aldrich	487052	p-Nitrophenyl-N-acetyl-β-D-glucosaminide - CAS 3459-18-5 - Calbiochem Useful substrate for rapid colorimetric assay of N-acetyl-β-glucosaminidase activity in human urine.	3459-18-5	1g	$229	2026-04-30	Buy
TCI Chemical	N0866	4-Nitrophenyl 2-Acetamido-2-deoxy-β-D-glucopyranoside >98.0%(HPLC)(N)	3459-18-5	200mg	$66	2026-04-30	Buy
TCI Chemical	N0866	4-Nitrophenyl 2-Acetamido-2-deoxy-β-D-glucopyranoside >98.0%(HPLC)(N)	3459-18-5	1g	$199	2026-04-30	Buy
Cayman Chemical	28954	4-Nitrophenyl-N-acetyl-β-D-glucosaminide ≥98%	3459-18-5	100mg	$49	2026-04-30	Buy

Product number	Packaging	Price	Buy
N9376	1g	$300	Buy
487052	1g	$229	Buy
N0866	200mg	$66	Buy
N0866	1g	$199	Buy
28954	100mg	$49	Buy

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide Chemical Properties,Uses,Production

Chemical Properties

Slightly Off-White Crystalline Solid

Uses

4-Nitrophenyl-N-acetyl-β- D-glucosaminide is a useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine

Uses

substrate for a-L-fucosidases

Uses

Useful substrate for the rapid colorimetric assay of N-Acetyl-beta-glucosaminidase in human urine.

Definition

ChEBI: An N-acetyl-beta-D-glucosaminide in which the anomeric hydroxy hydrogen is replaced by a 4-nitrophenyl group.

Biological Activity

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide Preparation Products And Raw materials

Raw materials

Preparation Products

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide Suppliers

Global( 282)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Jinan Samuel Pharmaceutical Co., Ltd.	0531-88821801 15192360884	605818151@qq.com	China	479	58
Feiyang Biotechnology Co., Ltd	0533-7866336 13573361699	sales@chemfy.com	China	524	62
Shanghai Yingxin laboratory equipment Co., Ltd	021-021-59178156 15300768757	359382369@qq.com	China	10667	58
Henan Evonik Biotechnology Co. LTD	16650615803	2224986086@qq.com	China	4986	58
Shanghai Fuorain BioTech Co., Ltd	18672534757	xiaoxue.hu@fuorain.cn	China	2039	58
Jinan Ruiyao Mingzhi Medical Technology Co., Ltd	15662630077 15662630077	958367155@qq.com	China	570	58
J & K SCIENTIFIC LTD.	18210857532 18210857532	jkinfo@jkchemical.com	China	96815	76
Meryer (Shanghai) Chemical Technology Co., Ltd.	4006356688 18621169109	market03@meryer.com	China	40202	62
3B Pharmachem (Wuhan) International Co.,Ltd.	18930552037	3bsc@sina.com	China	15813	69
TCI (Shanghai) Development Co., Ltd.	021-67121386	Sales-CN@TCIchemicals.com	China	24512	81

Supplier	Advantage
Jinan Samuel Pharmaceutical Co., Ltd.	58
Feiyang Biotechnology Co., Ltd	62
Shanghai Yingxin laboratory equipment Co., Ltd	58
Henan Evonik Biotechnology Co. LTD	58
Shanghai Fuorain BioTech Co., Ltd	58
Jinan Ruiyao Mingzhi Medical Technology Co., Ltd	58
J & K SCIENTIFIC LTD.	76
Meryer (Shanghai) Chemical Technology Co., Ltd.	62
3B Pharmachem (Wuhan) International Co.,Ltd.	69
TCI (Shanghai) Development Co., Ltd.	81

View Lastest Price from 4-Nitrophenyl-N-acetyl-beta-D-glucosaminide manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2026-09-11	4-Nitrophenyl N-acetyl-β-D-glucosaminide 3459-18-5		10G	98%min	30kg/month	WUHAN FORTUNA CHEMICAL CO., LTD
2026-08-25	4-Nitrophenyl 2-acetamido-2-deoxyhexopyranoside 3459-18-5	$2.20-8.80	1KG	99%		ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD
2019-07-06	4-Nitrophenyl 2-acetamido-2-deoxyhexopyranoside 3459-18-5	$1.00	1kg	95%-99%	1000kg	Career Henan Chemical Co

4-Nitrophenyl N-acetyl-β-D-glucosaminide
3459-18-5
$0.00
98%min
WUHAN FORTUNA CHEMICAL CO., LTD

4-Nitrophenyl 2-acetamido-2-deoxyhexopyranoside
3459-18-5
$2.20-8.80
99%
ZHENGZHOU JIUYI TIME NEW MATERIALS CO,.LTD

4-Nitrophenyl 2-acetamido-2-deoxyhexopyranoside
3459-18-5
$1.00
95%-99%
Career Henan Chemical Co

4-Nitrophenyl-N-acetyl-beta-D-glucosaminide Spectrum

4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(3459-18-5)MS 4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(3459-18-5)¹HNMR 4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(3459-18-5)IR1 4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(3459-18-5)IR2 4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE(3459-18-5)Raman

3459-18-5(4-Nitrophenyl-N-acetyl-beta-D-glucosaminide)Related Search:

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4-Nitrophenyl2-acetamido-2-deoxy-4-O-[2-O-(a-L-fucopyranosyl)-b-D-galactopyranosyl]-b-D-glucopyranoside 4-Nitrophenyl2-acetamido-3,6-di-O-acety-2-deoxyl-b-D-glucopyranoside Phenyl-N-acetyl-beta-D-glucosaminide 4-Nitrophenyl2-acetamido-2-deoxy-3-O-[2-O-(a-L-fucopyranosyl)-b-D-galactopyranosyl]-b-D-glucopyranoside 4-Nitrophenyl2-acetamido-2-deoxy-4,6-O-benzylidene-b-D-glucopyranoside

4-Nitrophenyl2-acetamido-3,6-di-O-acetyl-4-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-b-D-glucopyranoside p-Nitrophenyl beta-D-N,N',N''-triacetylchitotriose p-Nitrophenyl 6-sulfo-2-acetamido-2-deoxy-β-D-glucopyranoside, potassium salt

1of4

GLCNAC1-B-PNP 4-NITROPHENYL-N-ACETYL-B-D-GLUCOSAMINIDE 4-NITROPHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE (4'-NITRO)PHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE 4-NITROPHENYL-2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE 4-NITROPHENYL-2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOSIDE 4-NITROPHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOSIDE-(1,5) 4'-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside P-NITROPHENYL N-ACETYL-BETA-D-GLUCOSAMINIDE PNP N-ACETYL-BETA-D-GLUCOSAMINIDE PNP-BETA-D-GLCNAC P-NITROPHENYL 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSIDE P-NITROPHENYL ACETYL-BETA-D-GLUCOSAMINIDE P-NITROPHENYL-BETA-D-N-ACETYL-GLUCOSAMINIDE P-NITROPHENYL-N-ACETYL-B-D-*GLUCOSAMINID E P-Nitrophenyl-2-Acetamido-2-Deoxy-B-D- 1-(4-Nitrophenyl)-N-acetyl-beta-D-glucosaminide 4-Nitrophenyl 2-acetamido-2-deoxy- N-((2S,3R,4R,5S,6R)-4,5-Dihydroxy-6-(hydroxymethyl)-2-(4-nitrophenoxy)tetrahydro-2H-pyran-3-yl)acetamide p-Nitrophenyl 2-Acetamido-2-deoxy-b-D-glucopyranoside 4-nitrophenyl 2-acetamido-2-deoxy-β-d-glucopyranoside 4-Nitrophenyl-N-acetyl-bata-D-glucosaMinide 4-Nitrophenyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside 4-Nitrophenyl 2-acetamido-2-deoxyhexopyranoside p-Nitrophenyl-N-acetyl-β-D-glucosaminide - CAS 3459-18-5 - Calbiochem 4-Nitrophenyl N-acetyl-β-D-glucosaminide(PNP-NAG) 4-Nitrophenyl2-acetamido-2-deoxy-b-D-glucopyranoside 4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside 1-(4-Nitrophenyl)-N-acetyl-B-D-glucosaminide 2-NITROPHENYL-N-ACETYL-β-D-GLUCOSAMINE p-Nitrophenyl-b-D-N-acetyl-glucosaminide 1-O-(4-Nitrophenyl)-N-acetyl-β-D-glucosamine 1-O-(p-Nitrophenyl)-N-acetyl-β-D-glucosamine 4-Nitrophenyl 2-(acetylamino)-2-deoxy-β-D-glucopyranoside p-Nitrophenyl-N-acetylglucosaminide 4-Nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside,99% 4-nitrophenyl-N-acetyl-β-D-glucosaminide, p-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside, 4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside 4-Nitrophenyl N-acetyl-β-D-glucosaminide ,98% 4-Nitrophenyl N-acetyl-β-D-glucosaminide,4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside 4'-nitrophenyl-2-acetamido-2-deoxy-β-glucopyranoside 4-Nitrophenyl-2-acetaMido-2-deoxy-beta-D-glucopyranoside, 99% 100MG 4-Nitrophenyl 2-Acetamido-2-deoxy-β-D-glucopyranoside > 4-Nitorphenyl--N-acetyl-D- β-D-Glucopyranoside, 4-nitrophenyl 2-(acetylamino)-2-deoxy- 4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside 4-Nitrophenyl N-acetyl-β-D-glucosaminide 4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE USP/EP/BP p-Nitrophenyl 2-Acetamido-2-deoxy-β-D-glucopyranoside b-D-Glucopyranoside, 4-nitrophenyl 2-(acetylamino)-2-deoxy- 4-Nitrophenyl-N-acetyl-β-D-glucopyranoside 3459-18-5 4-NITROPHENYL-N-ACETYL-BETA-D-GLUCOSAMINIDE 4-Nitrophenyl-N-Acetyl-B-D-Glucosaminide extrapure, 99% 4-nitrophenyl- -D-N-acetyl-glucosaminide 4NitrophenylNacetylβDglucosaminide,4 Nitrophenyl N acetyl β D glucosaminide p-Nitrophenyl N-acetyl-β-D-glucosaminide 4-Nitrophenyl N-acetyl-β-D-glucosaminide p-Nitrophenyl-N-acetyl-β-D-glucosaminide 4-Nitrophenyl 2-acetamido-2-deoxy-β