HEPES sodium salt
- CAS No.
- 75277-39-3
- Chemical Name:
- HEPES sodium salt
- Synonyms
- HEPES NA;HEPES SODIUM SALT BUFFER;HEPES, SODIUM;H7006-500G;(HEPES)2-NA;HEPES NA-SALT;RUTHENIUM STAND;HEPES LOWSODIUM;HEPES SODIUM SALT;1.0 M HEPES sodium
- CBNumber:
- CB9773992
- Molecular Formula:
- C8H19N2NaO4S
- Molecular Weight:
- 261.3
- MDL Number:
- MFCD00036463
- MOL File:
- 75277-39-3.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 234 °C |
|---|---|
| Density | 1.504[at 20℃] |
| vapor pressure | 0Pa at 25℃ |
| storage temp. | room temp |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | dry powder |
| color | White |
| PH Range | 6.8 - 8.2 |
| PH | 10.0-12.0 (1M in H2O) |
| pka | 7.5(at 25℃) |
| biological source | synthetic |
| Water Solubility | It is soluble in water. |
| Sensitive | Hygroscopic |
| Stability | Stable. Hygroscopic - protect from moisture. Incompatible with strong oxidizing agents. |
| InChI | 1S/C8H18N2O4S.Na.H2O/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;;/h11H,1-8H2,(H,12,13,14);;1H2/q;+1;/p-1 |
| InChIKey | ZNJHFNUEQDVFCJ-UHFFFAOYSA-M |
| SMILES | O.[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O |
| CAS DataBase Reference | 75277-39-3(CAS DataBase Reference) |
| FDA UNII | Z9FTO91O8A |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt (75277-39-3) |
| Absorption |
≤0.2 at 260 in H2O at 1M ≤0.2 at 280 in H2O at 1M |
| UNSPSC Code | 12161704 |
| NACRES | NA.25 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H335 | |||||||||
| Precautionary statements | P280-P304+P340-P305+P351+P338 | |||||||||
| PPE | Eyeshields, Gloves, type N95 (US) | |||||||||
| Safety Statements | 22-24/25 | |||||||||
| WGK Germany | 3 | |||||||||
| F | 3-10 | |||||||||
| TSCA | TSCA listed | |||||||||
| HS Code | 29335995 | |||||||||
| Storage Class | 13 - Non Combustible Solids | |||||||||
| REACH Registrations | Active | |||||||||
| NFPA 704 |
|
HEPES sodium salt price More Price(90)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 391333 | HEPES, Sodium Salt, ULTROL® Grade A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.55 at 25°C. Absorbance (1.0 mM, H2O, 260 nm):0.10. | 75277-39-3 | 500G | $624 | 2026-04-30 | Buy |
| Sigma-Aldrich | RES6007H-A7 | HEPES sodium salt | 75277-39-3 | 1kg | $1141 | 2025-07-31 | Buy |
| Sigma-Aldrich | RES6007H-A7 | HEPES sodium salt | 75277-39-3 | 25kg | $14299 | 2025-07-31 | Buy |
| Sigma-Aldrich | 391333 | HEPES, Sodium Salt, ULTROL® Grade A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.55 at 25°C. Absorbance (1.0 mM, H2O, 260 nm):0.10. | 75277-39-3 | 100G | $166 | 2025-07-31 | Buy |
| Sigma-Aldrich | 391333 | HEPES, Sodium Salt, ULTROL® Grade A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.55 at 25°C. Absorbance (1.0 mM, H2O, 260 nm):0.10. | 75277-39-3 | 1KG | $900 | 2025-07-31 | Buy |
HEPES sodium salt Chemical Properties, Uses, Production
Chemical Properties
white powder
Uses
HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.
Definition
ChEBI: Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organic sodium salt, a N-(2-hydroxyethyl)piperazine and a N-(sulfoalkyl)piperazine. It contains a 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate.
General Description
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.
Flammability and Explosibility
Not classified
Biological Activity
Multi purpose buffer used in biological research.
Synthesis
103-76-4
1562-00-1
75277-39-3
The general procedure for the synthesis of N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium salt from N-hydroxyethylpiperazine and sodium 2-hydroxyethanesulfonate is as follows: magnetic stirring in a 70-mL fluoropolymer-lined steel autoclave equipped with internal thermocouples for monitoring the temperature of the liquid phase, a pressure gauge, a pressure-reducing valve, and an exhaust valve. First, amine feedstock, anhydrous or aqueous sodium 2-hydroxyethanesulfonate solution, and anhydrous or aqueous sodium hydroxide solution were added to the open-topped autoclave. Subsequently, the autoclave was assembled and immersed in an electrically heated oil bath. The stirred reaction mixture was rapidly heated to the specified reaction temperature with the exhaust valve closed and maintained at that temperature for the time specified in Examples 9, 10 and 13. Upon completion of the reaction, the mixture was cooled to room temperature, the reactor was opened, and the reaction product was dissolved in a sufficient amount of water to ensure complete dissolution of all solids.
storage
Room temperature
References
[1] Patent: US2006/89509, 2006, A1. Location in patent: Page/Page column 4-6
HEPES sodium salt Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| AILY INTL CHEMICAL CORP LTD | 025-83208295 18913388297 | ben@ailychem.com | China | 132 | 50 |
| Hunan Wokai Biotechnology Co., Ltd. | 0744-2221508 13126795102 | 2577930172@qq.com | China | 316 | 58 |
| Hubei XinghengKang Chemical Technology Co., Ltd. | 027-88878869 18162384755 | xhkhu2014@163.com | China | 240 | 58 |
| Hunan Hengfei bio-pharmaceutical Co. , Ltd. | 0746-6222194 13874779848 | 57182844@qq.com | China | 52 | 58 |
| Shanghai CanSpecsci Instrument Co., Ltd. | 400-6087598 15021221957 | order@canspecsci.com | China | 2001 | 56 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 18930552037 | 3bsc@sina.com | China | 15813 | 69 |
| future industrial shanghai co., ltd | 400-0066400 13621662912 | sales@jonln.com | China | 1989 | 65 |
| Alfa Aesar | 400-6106006 | saleschina@alfa-asia.com | China | 30103 | 84 |
View Latest Price from HEPES sodium salt manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-17 | HEPES sodium salt
75277-39-3
|
$5.00 | 1kg | ≥99% | 200mt/year | Jinan Finer Chemical Co., Ltd | |
 |
2026-05-28 | HEPES sodium salt
75277-39-3
|
$9.00 | 1KG | 99.8% | 100tons | Hebei Chuanghai Biotechnology Co,.LTD | |
 |
2025-06-27 | HEPES sodium salt
75277-39-3
|
1KG | 99% | 500000kg | Hebei Chuanghai Biotechnology Co., Ltd |
-
- HEPES sodium salt
75277-39-3
- $5.00
- ≥99%
- Jinan Finer Chemical Co., Ltd
-
- HEPES sodium salt
75277-39-3
- $9.00
- 99.8%
- Hebei Chuanghai Biotechnology Co,.LTD
-
- HEPES sodium salt
75277-39-3
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
