ビオチンHPDP 化学特性,用途語,生産方法
外観
白色～うすい黄色粉末～結晶
説明
Biotin-HPDP is a sulfhydryl-reactive biotinylation reagent that forms a reversible disulfide linkage. It is used to label protein cysteines and other substrates that contain sulfhydryl groups. Biotin-HPDP is also used in the biotin switch technique to tag S-nitrosylated (SNO) proteins, following reduction of SNO groups to thiols. Compounds that are tagged with biotin interact avidly with streptavidin-coupled beads, fluorophores, enzymes, etc. The interaction of biotin-HPDP with substrates containing sulfhydryl groups is easily performed at pH 6.5 to 7.5 in buffers such as PBS. The disulfide linkage that is formed between avidin and substrate can later by cleaved by a reducing agent, like dithiothreitol.
化学的特性
Pale Yellow Solid
使用
This compounds reacts specifically with thiol groups.
主な応用
Mustafa et al. applied S-methylmethanethiosulfonate (MMTS) and N-(6-(biotinamido)hexyl)-3′-(2′-pyridyldithio)propionamide (BIOTIN HPDP) to detect protein polysulfidation. The assumption was that MMTS exclusively blocks −SH, while BIOTIN HPDP can tag −SSH
[6].
ビオチンHPDP 上流と下流の製品情報
原材料
準備製品