Guanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid. It is a chaotropic agent, mainly involved in the extraction of DNA and RNA. It is utilized to deactivate the influenza virus.
일반 설명
Guanidine thiocyanate is chaotropic salts which lyse cells and along with nuclease binding matrix it is also seen to inhibit nuclease activity during extraction of DNA. It works as a strong RNase inhibitor in extraction buffers.
주요 응용
Guanidine thiocyanate is a chaotropic agent, protein denaturant and protector of DNA and RNA. The thiocyanate has an enzyme (nuclease) inhibiting function which, combined with its ability to protect nucleic acids, makes it useful in the study of DNA and RNA. It can be used to deactivate viruses so that they can be studied safely. It is used in PCR, Northern and Southern blotting.
